Anti-RhoF antibody (ab101349)
Key features and details
- Rabbit polyclonal to RhoF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RhoF antibody
See all RhoF primary antibodies -
Description
Rabbit polyclonal to RhoF -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Pig -
Immunogen
Recombinant fragment, corresponding to a region within internal sequence amino acids 16-156 of Human RhoF (AAH18208).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 24 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 24 kDa. |
Target
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Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. -
Cellular localization
Cell membrane. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 54509 Human
- Entrez Gene: 23912 Mouse
- SwissProt: Q9HBH0 Human
- SwissProt: Q8BYP3 Mouse
- Unigene: 644504 Human
- Unigene: 707579 Human
- Unigene: 720066 Human
- Unigene: 253876 Mouse
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Alternative names
- ARHF antibody
- FLJ20247 antibody
- Ras homolog gene family member F (in filopodia) antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab101349 has been referenced in 2 publications.
- Heib T et al. RhoA/Cdc42 signaling drives cytoplasmic maturation but not endomitosis in megakaryocytes. Cell Rep 35:109102 (2021). PubMed: 33979620
- Yang RM et al. miR-3656 expression enhances the chemosensitivity of pancreatic cancer to gemcitabine through modulation of the RHOF/EMT axis. Cell Death Dis 8:e3129 (2017). WB, IHC-P . PubMed: 29048402