Overview

  • Product name

  • Description

    Rabbit polyclonal to RhoF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 16-156 of Human RhoF (AAH18208).

  • Positive control

    • U87-MG whole cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal colon.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.

Target

  • Function

    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rho family.
  • Cellular localization

    Cell membrane. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARHF antibody
    • FLJ20247 antibody
    • Ras homolog gene family member F (in filopodia) antibody
    • Ras homolog gene family member F antibody
    • Rho F antibody
    • Rho family GTPase Rif antibody
    • Rho in filopodia antibody
    • Rho related GTP binding protein RhoF antibody
    • Rho-related GTP-binding protein RhoF antibody
    • Rhof antibody
    • RHOF_HUMAN antibody
    • RIF antibody
    see all

Images

  • IHC image of RhoF staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab101349, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-RhoF antibody (ab101349) at 1/500 dilution + U87-MG whole cell lysate at 30 µg

    Predicted band size: 24 kDa



    12% SDS PAGE

References

This product has been referenced in:

  • Yang RM  et al. miR-3656 expression enhances the chemosensitivity of pancreatic cancer to gemcitabine through modulation of the RHOF/EMT axis. Cell Death Dis 8:e3129 (2017). WB, IHC-P . Read more (PubMed: 29048402) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Answer

Thank you for your reply.

I am sorry that optimization hasn't helped improve the results. Would you like to receive a replacement vial as previous vials were working successfully?

Looking forward to your reply.

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Question
Answer

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab101349. I would also appreciate if you can confirm some further details:

- Use BSA instead of milk, this can sometimes give more satisfactory results and alter background signal.
- Incubate the primary antibody and membrane at room temperature for 6-8 hours. This can increase the level of specific antibody binding.
- Do you see different background in the IP'd and non-IP'd samples?
- What species and cell lines have you tested?



Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

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Answer

Vielen Dank für Ihren Anruf.

Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

Ich habe unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Fragebogens erhalten wir alle nötigen Informationen über Ihre Proben und Ihr Protokoll. Sobald Sie dieses Formular an uns zurückgeschickt haben, werden wir uns Ihr Protokoll ansehen, und dies gegebenfalls an das Labor weiterleiten. Desweiteren werde ich Ihnen dann wie abgesprochenden Ersatz zukommen lassen.




Ich freue mich, bald wieder von Ihnen zu hören.

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