Anti-RhoF antibody (ab191534)

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Western blot - Anti-RhoF antibody (ab191534)

    Key features and details

    • Rabbit polyclonal to RhoF
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-RhoF antibody
      See all RhoF primary antibodies

    • Description

      Rabbit polyclonal to RhoF

    • Host species

      Rabbit

    • Specificity

      ab191534 detects endogenous levels of RhoF.

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Mouse, Rat, Human

    • Immunogen

      Synthetic peptide corresponding to Human RhoF.
      Database link: Q9HBH0

    • Positive control

      • HeLa, mouse Raw 264.7 and rat H9C2 cell lysates.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituents: 50% Glycerol, 49% PBS
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      ab191534 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen, and the purity is > 95% (by SDS-PAGE)

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab191534 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.

    Target

    • Function

      Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.

    • Sequence similarities

      Belongs to the small GTPase superfamily. Rho family.

    • Cellular localization

      Cell membrane. Cytoplasm > cytoskeleton.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ARHF antibody
      • FLJ20247 antibody
      • Ras homolog gene family member F (in filopodia) antibody
      • Ras homolog gene family member F antibody
      • Rho F antibody
      • Rho family GTPase Rif antibody
      • Rho in filopodia antibody
      • Rho related GTP binding protein RhoF antibody
      • Rho-related GTP-binding protein RhoF antibody
      • Rhof antibody
      • RHOF_HUMAN antibody
      • RIF antibody
      see all

    Images

    • Western blot - Anti-RhoF antibody (ab191534)
      Western blot - Anti-RhoF antibody (ab191534)
      All lanes : Anti-RhoF antibody (ab191534)

      Lane 1 : HeLa whole cell lysate
      Lane 2 : mouse Raw 264.7 whole cell lysate
      Lane 3 : rat H9C2 whole cell lysate

      Predicted band size: 24 kDa

    References (0)

    Publishing research using ab191534? Please let us know so that we can cite the reference in this datasheet.

    ab191534 has not yet been referenced specifically in any publications.

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