- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-RhoF antibody
See all RhoF primary antibodies -
DescriptionRabbit polyclonal to RhoF
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Host speciesRabbit
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Specificityab191534 detects endogenous levels of RhoF.
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to Human RhoF.
Database link: Q9HBH0 -
Positive control
- HeLa, mouse Raw 264.7 and rat H9C2 cell lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab191534 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen, and the purity is > 95% (by SDS-PAGE)
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab191534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 24 kDa. |
Target
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FunctionPlasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.
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Sequence similaritiesBelongs to the small GTPase superfamily. Rho family.
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Cellular localizationCell membrane. Cytoplasm > cytoskeleton.
- Information by UniProt
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Database links
- Entrez Gene: 54509 Human
- Entrez Gene: 23912 Mouse
- Entrez Gene: 690130 Rat
- SwissProt: Q9HBH0 Human
- SwissProt: Q8BYP3 Mouse
- Unigene: 644504 Human
- Unigene: 707579 Human
- Unigene: 720066 Human
see all -
Alternative names
- ARHF antibody
- FLJ20247 antibody
- Ras homolog gene family member F (in filopodia) antibody
see all
Images
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Western blot - Anti-RhoF antibody (ab191534)All lanes : Anti-RhoF antibody (ab191534)
Lane 1 : HeLa whole cell lysate
Lane 2 : mouse Raw 264.7 whole cell lysate
Lane 3 : rat H9C2 whole cell lysate
Predicted band size: 24 kDa
Protocols
References
ab191534 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"