Anti-RhoF antibody (ab191534)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RhoF antibody
See all RhoF primary antibodies -
Description
Rabbit polyclonal to RhoF -
Host species
Rabbit -
Specificity
ab191534 detects endogenous levels of RhoF. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human RhoF.
Database link: Q9HBH0 -
Positive control
- HeLa, mouse Raw 264.7 and rat H9C2 cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab191534 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen, and the purity is > 95% (by SDS-PAGE) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab191534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 24 kDa. |
Target
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Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. -
Cellular localization
Cell membrane. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 54509 Human
- Entrez Gene: 23912 Mouse
- Entrez Gene: 690130 Rat
- SwissProt: Q9HBH0 Human
- SwissProt: Q8BYP3 Mouse
- Unigene: 644504 Human
- Unigene: 707579 Human
- Unigene: 720066 Human
see all -
Alternative names
- ARHF antibody
- FLJ20247 antibody
- Ras homolog gene family member F (in filopodia) antibody
see all
Images
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Western blot - Anti-RhoF antibody (ab191534)All lanes : Anti-RhoF antibody (ab191534)
Lane 1 : HeLa whole cell lysate
Lane 2 : mouse Raw 264.7 whole cell lysate
Lane 3 : rat H9C2 whole cell lysate
Predicted band size: 24 kDa
Protocols
References
ab191534 has not yet been referenced specifically in any publications.
