Product nameAnti-RhoGDI antibody [EPR3772]
See all RhoGDI primary antibodies
DescriptionRabbit monoclonal [EPR3772] to RhoGDI
Tested applicationsSuitable for: WB, ICCmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human RhoGDI aa 1-100. The exact sequence is proprietary.
- Jurkat, HeLa, HL-60 and NIH/3T3 cell lysates
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).|
|ICC||1/50 - 1/100.|
FunctionRegulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Sequence similaritiesBelongs to the Rho GDI family.
- Information by UniProt
- ARHGDIA antibody
- fa96g11 antibody
- GDIA 1 antibody
All lanes : Anti-RhoGDI antibody [EPR3772] (ab108977) at 1/1000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : HL-60 cell lysates
Lane 4 : NIH/3T3 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Kempf SJ et al. The cognitive defects of neonatally irradiated mice are accompanied by changed synaptic plasticity, adult neurogenesis and neuroinflammation. Mol Neurodegener 9:57 (2014). WB . Read more (PubMed: 25515237) »
- Yentrapalli R et al. The PI3K/Akt/mTOR pathway is implicated in the premature senescence of primary human endothelial cells exposed to chronic radiation. PLoS One 8:e70024 (2013). Human . Read more (PubMed: 23936371) »