Overview

  • Product name

    Anti-RhoGDI antibody [EPR3772]
    See all RhoGDI primary antibodies
  • Description

    Rabbit monoclonal [EPR3772] to RhoGDI
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RhoGDI aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Jurkat, HeLa, HL-60 and NIH/3T3 cell lysates
  • General notes

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
    • Sequence similarities

      Belongs to the Rho GDI family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • ARHGDIA antibody
      • fa96g11 antibody
      • GDIA 1 antibody
      • GDIA1 antibody
      • GDIR1_HUMAN antibody
      • MGC117248 antibody
      • NPHS8 antibody
      • Rho GDI 1 antibody
      • Rho GDI alpha antibody
      • Rho GDI antibody
      • Rho GDP dissociation inhibitor (GDI) alpha antibody
      • Rho GDP dissociation inhibitor 1 antibody
      • Rho GDP dissociation inhibitor alpha antibody
      • Rho GDP-dissociation inhibitor 1 antibody
      • Rho-GDI alpha antibody
      • RhoGDI 1 antibody
      • RhoGDI alpha antibody
      • RHOGDI antibody
      • RhoGDI1 antibody
      • wu:fa96g11 antibody
      • zgc:55554 antibody
      • zgc:77681 antibody
      see all

    Images

    • All lanes : Anti-RhoGDI antibody [EPR3772] (ab108977) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : HL-60 cell lysates
      Lane 4 : NIH/3T3 cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 23 kDa
      Observed band size: 26 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Kempf SJ  et al. The cognitive defects of neonatally irradiated mice are accompanied by changed synaptic plasticity, adult neurogenesis and neuroinflammation. Mol Neurodegener 9:57 (2014). WB . Read more (PubMed: 25515237) »
    • Yentrapalli R  et al. The PI3K/Akt/mTOR pathway is implicated in the premature senescence of primary human endothelial cells exposed to chronic radiation. PLoS One 8:e70024 (2013). Human . Read more (PubMed: 23936371) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for your email.

    Unfortunately the order is out of Abpromise guarantee period. The order was placed on 17th January 2012, which means it was under 6 month guarantee period until 16th June however the complaint was submitted in August so I am sorry we will not be able to replace the product on this occasion. I would suggest contacting us within 6 month period for claiming the guarantee period.

    We now have new lot of this product in stock. Please buy the new lot and use it as per your convenience, if in any case the product fails please let us know we will be happy to replace the product.

    I hope this information is nevertheless helpful to you. Should you have any question please do not hesitate to contact me.

    Read More

    Answer

    Thanks you for your email. I apologize for the delay.

    We unfortunately are unable to find reason why the activity of this antibody is dropping. Also we haven't received any other quality complaint for this antibody so I am sorry we will not be retesting this product at the moment.

    To proceed further in this case; could you please provide the purchase order number? I assume that the product you have purchase is within 6 month guarantee period and might be eligible for replacement. If the product was purchased within 6 month guarantee period I will be happy replacing the product.

    I will look forward to receiving the order number soon.

    Read More

    Question
    Answer

    Thank you very much for sending the information.

    In order to understand the problem better could you provide details how this antibody was stored?

    Was the antibody degradation gradual or it just stopped working after 6 months?

    Many thanks for your cooperation in advance. I will look forward to hearing from you soon.

    Read More

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