Overview

  • Product name

    Anti-RhoGDI antibody [EPR3773]
    See all RhoGDI primary antibodies
  • Description

    Rabbit monoclonal [EPR3773] to RhoGDI
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues within human RhoGDI (P52565).

  • Positive control

    • Jurkat; HeLa and NIH3T3 cell lysates. Human breast carcinoma tissue.
  • General notes

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
    • Sequence similarities

      Belongs to the Rho GDI family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • ARHGDIA antibody
      • fa96g11 antibody
      • GDIA 1 antibody
      • GDIA1 antibody
      • GDIR1_HUMAN antibody
      • MGC117248 antibody
      • NPHS8 antibody
      • Rho GDI 1 antibody
      • Rho GDI alpha antibody
      • Rho GDI antibody
      • Rho GDP dissociation inhibitor (GDI) alpha antibody
      • Rho GDP dissociation inhibitor 1 antibody
      • Rho GDP dissociation inhibitor alpha antibody
      • Rho GDP-dissociation inhibitor 1 antibody
      • Rho-GDI alpha antibody
      • RhoGDI 1 antibody
      • RhoGDI alpha antibody
      • RHOGDI antibody
      • RhoGDI1 antibody
      • wu:fa96g11 antibody
      • zgc:55554 antibody
      • zgc:77681 antibody
      see all

    Images

    • All lanes : Anti-RhoGDI antibody [EPR3773] (ab133248) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : NIH3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 23 kDa

    • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling RhoGDI with ab133248 at 1/100 dilution.

    References

    This product has been referenced in:

    • Lai MC  et al. Prognostic value of Rho GDP dissociation inhibitors in patients with hepatocellular carcinoma following liver transplantation. Oncol Lett 14:1395-1402 (2017). Read more (PubMed: 28789355) »
    • Huang D  et al. Rho GDP-dissociation inhibitor a is a potential prognostic biomarker and controls telomere regulation in colorectal cancer. Cancer Sci 108:1293-1302 (2017). WB ; Human . Read more (PubMed: 28417530) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

    Abcam user community

    Verified customer

    Submitted Jun 22 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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