Product nameAnti-RhoGDI antibody [EPR3773]
See all RhoGDI primary antibodies
DescriptionRabbit monoclonal [EPR3773] to RhoGDI
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
A synthetic peptide corresponding to residues within human RhoGDI (P52565).
- Jurkat; HeLa and NIH3T3 cell lysates. Human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 23 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/50 - 1/100.|
FunctionRegulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Sequence similaritiesBelongs to the Rho GDI family.
- Information by UniProt
- ARHGDIA antibody
- fa96g11 antibody
- GDIA 1 antibody
All lanes : Anti-RhoGDI antibody [EPR3773] (ab133248) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling RhoGDI with ab133248 at 1/100 dilution.
This product has been referenced in:
- Lai MC et al. Prognostic value of Rho GDP dissociation inhibitors in patients with hepatocellular carcinoma following liver transplantation. Oncol Lett 14:1395-1402 (2017). Read more (PubMed: 28789355) »
- Huang D et al. Rho GDP-dissociation inhibitor a is a potential prognostic biomarker and controls telomere regulation in colorectal cancer. Cancer Sci 108:1293-1302 (2017). WB ; Human . Read more (PubMed: 28417530) »