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Signal Transduction Cytoskeleton / ECM Extracellular Matrix Structures Focal Adhesions
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Anti-RhoGDI (phospho S174) antibody (ab74142)

  • Datasheet
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Western blot - Anti-RhoGDI (phospho S174) antibody (ab74142)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGDI (phospho S174) antibody (ab74142)

Key features and details

  • Rabbit polyclonal to RhoGDI (phospho S174)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human RhoGDI protein (ab132108)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-RhoGDI (phospho S174) antibody
    See all RhoGDI primary antibodies
  • Description

    Rabbit polyclonal to RhoGDI (phospho S174)
  • Host species

    Rabbit
  • Specificity

    The antibody detects endogenous levels of RhoGDI only when phosphorylated at serine 174.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human RhoGDI around the phosphorylation site of serine 174 (R-G-SP-Y-S).

  • Positive control

    • IHC-P: Human cervix carcinoma tissue. WB: HUVEC cells treated with EGF.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was purified by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Focal Adhesions
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Regulators

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human RhoGDI protein (ab132108)

Applications

Our Abpromise guarantee covers the use of ab74142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 23 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function

    Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
  • Sequence similarities

    Belongs to the Rho GDI family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P52565 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 396 Human
    • Entrez Gene: 192662 Mouse
    • Entrez Gene: 360678 Rat
    • Omim: 601925 Human
    • SwissProt: P52565 Human
    • SwissProt: Q99PT1 Mouse
    • SwissProt: Q5XI73 Rat
    • Unigene: 159161 Human
    • Unigene: 30016 Mouse
    • Unigene: 474783 Mouse
    • Unigene: 1801 Rat
    see all
  • Alternative names

    • ARHGDIA antibody
    • fa96g11 antibody
    • GDIA 1 antibody
    • GDIA1 antibody
    • GDIR1_HUMAN antibody
    • MGC117248 antibody
    • NPHS8 antibody
    • Rho GDI 1 antibody
    • Rho GDI alpha antibody
    • Rho GDI antibody
    • Rho GDP dissociation inhibitor (GDI) alpha antibody
    • Rho GDP dissociation inhibitor 1 antibody
    • Rho GDP dissociation inhibitor alpha antibody
    • Rho GDP-dissociation inhibitor 1 antibody
    • Rho-GDI alpha antibody
    • RhoGDI 1 antibody
    • RhoGDI alpha antibody
    • RHOGDI antibody
    • RhoGDI1 antibody
    • wu:fa96g11 antibody
    • zgc:55554 antibody
    • zgc:77681 antibody
    see all

Images

  • Western blot - Anti-RhoGDI (phospho S174) antibody (ab74142)
    Western blot - Anti-RhoGDI (phospho S174) antibody (ab74142)
    All lanes : Anti-RhoGDI (phospho S174) antibody (ab74142) at 1/500 dilution

    Lane 1 : HUVEC cells treated with EGF (200ng/ml, 30mins)
    Lane 2 : HUVEC cells treated with EGF (200ng/ml, 30mins) with phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa
    Additional bands at: 105 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGDI (phospho S174) antibody (ab74142)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGDI (phospho S174) antibody (ab74142)
    Human cervix carcinoma tissue stained with ab74142 (1/50 dilution).
    1: No phosphopeptide
    2: Phosphopeptide

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab74142? Please let us know so that we can cite the reference in this datasheet.

    ab74142 has been referenced in 2 publications.

    • Keller-Pinter A  et al. The phosphomimetic mutation of syndecan-4 binds and inhibits Tiam1 modulating Rac1 activity in PDZ interaction-dependent manner. PLoS One 12:e0187094 (2017). PubMed: 29121646
    • Oishi A  et al. Regulation of RhoA Signaling by the cAMP-dependent Phosphorylation of RhoGDIa. J Biol Chem 287:38705-15 (2012). WB . PubMed: 23012358

    Customer reviews and Q&As

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