Key features and details
- Rabbit polyclonal to RhoGDI (phospho S174)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RhoGDI (phospho S174) antibody
See all RhoGDI primary antibodies
DescriptionRabbit polyclonal to RhoGDI (phospho S174)
SpecificityThe antibody detects endogenous levels of RhoGDI only when phosphorylated at serine 174.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthesized phosphopeptide derived from human RhoGDI around the phosphorylation site of serine 174 (R-G-SP-Y-S).
- IHC-P: Human cervix carcinoma tissue. WB: HUVEC cells treated with EGF.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was purified by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab74142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 23 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionRegulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Sequence similaritiesBelongs to the Rho GDI family.
- Information by UniProt
- ARHGDIA antibody
- fa96g11 antibody
- GDIA 1 antibody
All lanes : Anti-RhoGDI (phospho S174) antibody (ab74142) at 1/500 dilution
Lane 1 : HUVEC cells treated with EGF (200ng/ml, 30mins)
Lane 2 : HUVEC cells treated with EGF (200ng/ml, 30mins) with phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 105 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Human cervix carcinoma tissue stained with ab74142 (1/50 dilution).
1: No phosphopeptide
ab74142 has been referenced in 2 publications.
- Keller-Pinter A et al. The phosphomimetic mutation of syndecan-4 binds and inhibits Tiam1 modulating Rac1 activity in PDZ interaction-dependent manner. PLoS One 12:e0187094 (2017). PubMed: 29121646
- Oishi A et al. Regulation of RhoA Signaling by the cAMP-dependent Phosphorylation of RhoGDIa. J Biol Chem 287:38705-15 (2012). WB . PubMed: 23012358