Anti-RhoGDI (phospho S174) antibody (ab74142)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-RhoGDI (phospho S174) antibody
See all RhoGDI primary antibodies -
Description
Rabbit polyclonal to RhoGDI (phospho S174) -
Host species
Rabbit -
Specificity
The antibody detects endogenous levels of RhoGDI only when phosphorylated at serine 174. -
Tested applications
Suitable for: WB, ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthesized phosphopeptide derived from human RhoGDI around the phosphorylation site of serine 174 (R-G-SP-Y-S).
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Positive control
- IHC-P: Human cervix carcinoma tissue. WB: HUVEC cells treated with EGF.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was purified by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab74142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 23 kDa. | |
| ELISA | 1/20000. | |
| IHC-P | 1/50 - 1/100. |
Target
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Function
Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. -
Sequence similarities
Belongs to the Rho GDI family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 396 Human
- Entrez Gene: 192662 Mouse
- Entrez Gene: 360678 Rat
- Omim: 601925 Human
- SwissProt: P52565 Human
- SwissProt: Q99PT1 Mouse
- SwissProt: Q5XI73 Rat
- Unigene: 159161 Human
see all -
Alternative names
- ARHGDIA antibody
- fa96g11 antibody
- GDIA 1 antibody
see all
Images
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Western blot - Anti-RhoGDI (phospho S174) antibody (ab74142)All lanes : Anti-RhoGDI (phospho S174) antibody (ab74142) at 1/500 dilution
Lane 1 : HUVEC cells treated with EGF (200ng/ml, 30mins)
Lane 2 : HUVEC cells treated with EGF (200ng/ml, 30mins) with phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 105 kDa, 60 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGDI (phospho S174) antibody (ab74142)Human cervix carcinoma tissue stained with ab74142 (1/50 dilution).
1: No phosphopeptide
2: Phosphopeptide
Datasheets and documents
References
This product has been referenced in:
- Keller-Pinter A et al. The phosphomimetic mutation of syndecan-4 binds and inhibits Tiam1 modulating Rac1 activity in PDZ interaction-dependent manner. PLoS One 12:e0187094 (2017). Read more (PubMed: 29121646) »
- Oishi A et al. Regulation of RhoA Signaling by the cAMP-dependent Phosphorylation of RhoGDIa. J Biol Chem 287:38705-15 (2012). WB . Read more (PubMed: 23012358) »
