• Product name
  • Description
    Rabbit polyclonal to RHOH
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide KLH conjugated corresponding to a region from the C terminus of Human RHOH

  • Positive control
    • Human colon and small intestine tissue. HT-29 cell lysate and A549 cells.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab118507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/500.


  • Function
    Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells (By similarity). Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils.
  • Tissue specificity
    Expressed only in hematopoietic cells. Present at very high levels in the thymus, less abundant in the spleen, and least abundant in the bone marrow. Expressed at a higher level in the TH1 subtype of T-helper cells than in the TH2 subpopulation. Expressed in neutrophils under inflammatory conditions, such as cystic fibrosis, ulcerative colitis and appendicitis.
  • Involvement in disease
    Note=A chromosomal aberration involving RHOH is found in a non-Hodgkin lymphoma cell line. Translocation t(3;4)(q27;p11) with BCL6.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rho family.
  • Domain
    The region involved in interaction with ZAP70 is a non-canonical immunoreceptor tyrosine-based activation motif (ITAM).
  • Post-translational
    Phosphorylated on tyrosine by LCK. Phosphorylated by FYN. Phosphorylation enhances the interactions with ZAP70 and SYK and is critical for its function in thymocyte development.
  • Cellular localization
    Cytoplasm. Cell membrane. Co-localizes together with ZAP70 in the immunological synapse.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHH antibody
    • GTP binding protein TTF antibody
    • GTP-binding protein TTF antibody
    • OTTHUMP00000125213 antibody
    • Ras homolog gene family member H antibody
    • Rho related GTP binding protein antibody
    • Rho related GTP binding protein RhoH antibody
    • Rho-related GTP-binding protein RhoH antibody
    • RHOH antibody
    • RHOH_HUMAN antibody
    • Translocation three four protein antibody
    • TTF antibody
    • TTF translocation three four antibody
    see all


  • ab118507 at 5 µg/ml staining RHOH in Formalin-Fixed, Paraffin-Embedded Human colon tissue.
  • ab118507 at 5 µg/ml staining RHOH in Formalin-Fixed, Paraffin-Embedded Human Small Intestine tissue.
  • All lanes : Anti-RHOH antibody (ab118507) at 1/500 dilution

    Lane 1 : HT-29 extract without the synthesized peptide.
    Lane 2 : HT-29 extract with the synthesized peptide.

    Predicted band size: 21 kDa

  • Immunofluorescence analysis of A549 cells, using ab118507 at 1/100. The picture on the right is treated with the synthesized peptide.


ab118507 has not yet been referenced specifically in any publications.

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