Key features and details
- Rabbit polyclonal to Rhp51
- Suitable for: WB, IP, ICC/IF
- Reacts with: Schizosaccharomyces pombe
- Isotype: IgG
Product nameAnti-Rhp51 antibody
DescriptionRabbit polyclonal to Rhp51
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Schizosaccharomyces pombe
Purified recombinant full length protein (Schizosaccharomyces pombe)
- Schizosaccharomyces pombe whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab63799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 40 kDa).|
|IP||Use at an assay dependent concentration.|
RelevanceRhp51 is required both for recombination and for the repair of DNA damage caused by X-rays. Binds to single and double stranded DNA, in the presence of magnesium, and exhibits DNA-dependent ATPase activity. Promotes DNA strand annealing and strand exchange via DNA recombinase activity and forms helical nucleoprotein filaments.
- DNA repair protein rhp51 antibody
- Rad51 antibody
- RAD51 homolog antibody
- Recombinase Rhp51 antibody
ab63799 (1/500) staining Rhp51 in S. pombe cells without or with MMS (0.025%) treatment for 1 hour by ICC/IF (Immunocytochemistry/immunofluorescence).
All lanes : Anti-Rhp51 antibody (ab63799) at 1/2000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Whole cell extract of Schizosaccharomyces pombe wild-type strain
Lane 3 : Whole cell extract of Schizosaccharomyces pombe Rad51 deletion strain
Predicted band size: 40 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
ab63799 has not yet been referenced specifically in any publications.