Product nameAnti-RIAM antibody [EPR2806]
See all RIAM primary antibodies
DescriptionRabbit monoclonal [EPR2806] to RIAM
Tested applicationsSuitable for: WB, IP, ICC, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human RIAM aa 1-100 (N terminal). The exact sequence is proprietary.
- Human Lymph node, Fetal brain, Human spleen, Jurkat and HL-60 + retinoic acid cell lysates; Human tonsil tissue
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab92537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
Is unsuitable for Flow Cyt.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceRIAM (Rap1-GTP interacting adapter molecule) appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling via interaction with profilin and VASP. It suppresses insulin-induced promoter activities through AP1 and SRE and also mediates Rap1-induced adhesion.
Cellular localizationCell Membrane, Cytoplasm and cytoskeleton
- AB1IP antibody
- Amyloid beta A4 precursor protein binding family B member 1 interacting protein antibody
- Amyloid beta precursor protein binding family B member 1 antibody
All lanes : Anti-RIAM antibody [EPR2806] (ab92537) at 1/2000 dilution
Lane 1 : Human Lymph node lysate
Lane 2 : Fetal brain lysate
Lane 3 : Human spleen lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HL-60 + retinoic acid cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 73 kDa
ab92537, at 1/100 dilution, staining RIAM in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product has been referenced in:
- Zhu L et al. Structure of Rap1b bound to talin reveals a pathway for triggering integrin activation. Nat Commun 8:1744 (2017). Read more (PubMed: 29170462) »
- Su W et al. Rap1 and its effector RIAM are required for lymphocyte trafficking. Blood 126:2695-703 (2015). Read more (PubMed: 26324702) »