Recombinant Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20357] to Ribonuclease 3/ECP
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Ribonuclease 3/ECP antibody [EPR20357]
See all Ribonuclease 3/ECP primary antibodies -
Description
Rabbit monoclonal [EPR20357] to Ribonuclease 3/ECP -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: GST-His-tagged human Ribonuclease 3/ECP recombinant protein (aa28-160); Human fetal lung and fetal spleen lysates. IHC-P: Human Hodgkin’s lymphoma, spleen and colon tissues.
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General notes
This product was previously labelled as Ribonuclease 3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20357 -
Isotype
IgG
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab207429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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WB |
Human
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Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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|
WB |
1/1000. Detects a band of approximately 16-18 kDa (predicted molecular weight: 18 kDa).
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Notes |
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IHC-P
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 16-18 kDa (predicted molecular weight: 18 kDa). |
Target
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Function
Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity. -
Sequence similarities
Belongs to the pancreatic ribonuclease family. -
Cellular localization
Cytoplasmic granule. Matrix of eosinophil's large specific granule. - Information by UniProt
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Database links
- Entrez Gene: 6037 Human
- Omim: 131398 Human
- SwissProt: P12724 Human
- Unigene: 73839 Human
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Alternative names
- Cytotoxic ribonuclease antibody
- ECP antibody
- ECP_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429)
Immunohistochemical analysis of paraffin-embedded human Hodgkin’s lymphoma tissue labeling Ribonuclease 3/ECP with ab207429 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on eosinophils and neutrophils of human Hodgkin’s lymphoma is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429) at 1/10000 dilution
Lane 1 : GST-His-tagged human Ribonuclease 3/ECP recombinant protein (aa28-160)
Lane 2 : GST-His-tagged human Ribonuclease 2 recombinant protein (aa28-160)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 4 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Ribonuclease 3/ECP with ab207429 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on eosinophils and neutrophils of human spleen [PMID: 11034418].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 16-18 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 2 seconds; Lane 2: 3 seconds.
The observed molecular weight is consistent with PMID:23597768, 19692640.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Ribonuclease 3/ECP with ab207429 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on eosinophils and neutrophils of human colon [PMID: 11034418].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab207429 has not yet been referenced specifically in any publications.