Recombinant
RabMAb

Recombinant Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429)

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Overview

  • Product name

    Anti-Ribonuclease 3/ECP antibody [EPR20357]
    See all Ribonuclease 3/ECP primary antibodies

  • Description

    Rabbit monoclonal [EPR20357] to Ribonuclease 3/ECP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein within Human Ribonuclease 3/ECP aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P12724

  • Positive control

    • WB: GST-His-tagged human Ribonuclease 3/ECP recombinant protein (aa28-160); Human fetal lung and fetal spleen lysates. IHC-P: Human Hodgkin’s lymphoma, spleen and colon tissues.

  • General notes

     This product was previously labelled as Ribonuclease 3

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20357

  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab207429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 16-18 kDa (predicted molecular weight: 18 kDa).

Target

  • Function

    Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity.

  • Sequence similarities

    Belongs to the pancreatic ribonuclease family.

  • Cellular localization

    Cytoplasmic granule. Matrix of eosinophil's large specific granule.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cytotoxic ribonuclease antibody
      • ECP antibody
      • ECP_HUMAN antibody
      • Eosinophil cationic protein antibody
      • OTTHUMP00000164017 antibody
      • Ribonuclease 3 antibody
      • Ribonuclease, RNase A family, 3 antibody
      • RNase 3 antibody
      • RNASE3 antibody
      • RNS3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429)

      Immunohistochemical analysis of paraffin-embedded human Hodgkin’s lymphoma tissue labeling Ribonuclease 3/ECP with ab207429 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on eosinophils and neutrophils of human Hodgkin’s lymphoma is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429)
      Western blot - Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429)
      All lanes : Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429) at 1/10000 dilution

      Lane 1 : GST-His-tagged human Ribonuclease 3/ECP recombinant protein (aa28-160)
      Lane 2 : GST-His-tagged human Ribonuclease 2 recombinant protein (aa28-160)

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 18 kDa
      Observed band size: 40 kDa
      why is the actual band size different from the predicted?


      Exposure time: 4 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429)

      Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Ribonuclease 3/ECP with ab207429 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on eosinophils and neutrophils of human spleen [PMID: 11034418].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429)
      Western blot - Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429)
      All lanes : Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429) at 1/1000 dilution

      Lane 1 : Human fetal lung lysate
      Lane 2 : Human fetal spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 18 kDa
      Observed band size: 16-18 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure times: Lane 1: 2 seconds; Lane 2: 3 seconds.

      The observed molecular weight is consistent with PMID:23597768, 19692640.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] (ab207429)

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Ribonuclease 3/ECP with ab207429 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on eosinophils and neutrophils of human colon [PMID: 11034418].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    References

    ab207429 has not yet been referenced specifically in any publications.

    Publishing research using ab207429? Please let us know so that we can cite the reference in this datasheet.

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