Recombinant
RabMAb

Recombinant Anti-Ribonuclease 3/ECP antibody [EPR20357] - BSA and Azide free (ab223148)

Overview

  • Product name

    Anti-Ribonuclease 3/ECP antibody [EPR20357] - BSA and Azide free
    See all Ribonuclease 3/ECP primary antibodies
  • Description

    Rabbit monoclonal [EPR20357] to Ribonuclease 3/ECP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P12724

  • Positive control

    • WB: GST-His-tagged human Ribonuclease 3/ECP recombinant protein (aa28-160); Human fetal lung and fetal spleen lysates. IHC-P: Human Hodgkin’s lymphoma, spleen and colon tissues.
  • General notes

    ab223148 is the carrier-free version of ab207429 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab223148 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Ribonuclease 3

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20357
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab223148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 16-18 kDa (predicted molecular weight: 18 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity.
  • Sequence similarities

    Belongs to the pancreatic ribonuclease family.
  • Cellular localization

    Cytoplasmic granule. Matrix of eosinophil's large specific granule.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytotoxic ribonuclease antibody
    • ECP antibody
    • ECP_HUMAN antibody
    • Eosinophil cationic protein antibody
    • OTTHUMP00000164017 antibody
    • Ribonuclease 3 antibody
    • Ribonuclease, RNase A family, 3 antibody
    • RNase 3 antibody
    • RNASE3 antibody
    • RNS3 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human Hodgkin’s lymphoma tissue labeling Ribonuclease 3/ECP with ab207429 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on eosinophils and neutrophils of human Hodgkin’s lymphoma is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207429).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Ribonuclease 3/ECP with ab207429 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on eosinophils and neutrophils of human spleen [PMID: 11034418].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207429).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Ribonuclease 3/ECP with ab207429 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on eosinophils and neutrophils of human colon [PMID: 11034418].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207429).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab223148 has not yet been referenced specifically in any publications.

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