Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab92876)
Key features and details
- Mouse polyclonal to Ribonuclease H2, subunit A/RNASEH2A
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Ribonuclease H2, subunit A/RNASEH2A antibody
See all Ribonuclease H2, subunit A/RNASEH2A primary antibodies -
Description
Mouse polyclonal to Ribonuclease H2, subunit A/RNASEH2A -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human Ribonuclease H2, subunit A/RNASEH2A. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_006388.2 -
Positive control
- COLO 320 HSR cell lysate, Jurkat cell lysate and HeLa cell.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 33 kDa.
|
|
ICC/IF |
Use a concentration of 10 µg/ml.
|
Notes |
---|
WB
1/500 - 1/1000. Predicted molecular weight: 33 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
-
Function
Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes. -
Involvement in disease
Aicardi-Goutieres syndrome 4 -
Sequence similarities
Belongs to the RNase HII family. Eukaryotic subfamily. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 10535 Human
- SwissProt: O75792 Human
- Unigene: 532851 Human
-
Alternative names
- AGS4 antibody
- Aicardi Goutieres syndrome 4 protein antibody
- Aicardi-Goutieres syndrome 4 protein antibody
see all
Images
-
Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab92876) at 1/500 dilution + COLO 320 HSR at 50 µg
Predicted band size: 33 kDa -
Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab92876) at 1/500 dilution + Jurkat cell lysate at 50 µg
Predicted band size: 33 kDa -
All lanes : Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab92876) at 1/500 dilution
Lane 1 : Ribonuclease H2, subunit A/RNASEH2A transfected 293T cell lysate
Lane 2 : Non-transfected cell line.
Lysates/proteins at 50 µg per lane.
Predicted band size: 33 kDa -
ab92876 at 10µg/ml staining Ribonuclease H2, subunit A/RNASEH2A on Hela cell by immunofluorescence
Protocols
Datasheets and documents
-
Datasheet download
References (3)
ab92876 has been referenced in 3 publications.
- Zimmermann M et al. CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Nature 559:285-289 (2018). PubMed: 29973717
- Kennedy EM et al. Frequent Incorporation of Ribonucleotides during HIV-1 Reverse Transcription and Their Attenuated Repair in Macrophages. J Biol Chem 287:14280-8 (2012). WB . PubMed: 22383524
- Helmrich A et al. Collisions between replication and transcription complexes cause common fragile site instability at the longest human genes. Mol Cell 44:966-77 (2011). PubMed: 22195969