Key features and details
- Rabbit polyclonal to Ribonuclease H2, subunit A/RNASEH2A
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Ribonuclease H2, subunit A/RNASEH2A antibody
See all Ribonuclease H2, subunit A/RNASEH2A primary antibodies
DescriptionRabbit polyclonal to Ribonuclease H2, subunit A/RNASEH2A
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Pig
Recombinant fragment within Human Ribonuclease H2, subunit A/RNASEH2A (internal sequence). The exact sequence is proprietary.
Database link: O75792
- WB: HeLa whole cell and nuclear extracts. ICC/IF: HeLa cells.
This product was previously labelled as Ribonuclease H2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 33 kDa.|
|ICC/IF||1/100 - 1/1000.|
RelevanceOf the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.
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4% paraformaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ribonuclease H2, subunit A/RNASEH2A (green) using ab228788 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue). Phalloidin, a cytoskeleton marker, was labeled 1/200 dilution.
All lanes : Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab228788) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa
12% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228788 has not yet been referenced specifically in any publications.