Overview

  • Product name

    Anti-Ribonuclease H2, subunit A/RNASEH2A antibody
    See all Ribonuclease H2, subunit A/RNASEH2A primary antibodies
  • Description

    Rabbit polyclonal to Ribonuclease H2, subunit A/RNASEH2A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant fragment within Human Ribonuclease H2, subunit A/RNASEH2A (internal sequence). The exact sequence is proprietary.
    Database link: O75792

  • Positive control

    • WB: HeLa whole cell and nuclear extracts. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as Ribonuclease H2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab228788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • AGS4 antibody
    • Aicardi Goutieres syndrome 4 protein antibody
    • EC 3.1.26.4 antibody
    • JUNB antibody
    • Ribonuclease HI large subunit antibody
    • Ribonuclease HI subunit A antibody
    • RNase H(35) antibody
    • RNase H2 subunit A antibody
    • RNase HI large subunit antibody
    • RNASEH2A antibody
    • RNASEHI antibody
    • RNHIA antibody
    • RNHL antibody
    see all

Images

  • 4% paraformaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ribonuclease H2, subunit A/RNASEH2A (green) using ab228788 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue). Phalloidin, a cytoskeleton marker, was labeled 1/200 dilution.

  • All lanes : Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab228788) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 33 kDa



    12% SDS-PAGE gel.

References

ab228788 has not yet been referenced specifically in any publications.

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