Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab83943)
Key features and details
- Rabbit polyclonal to Ribonuclease H2, subunit A/RNASEH2A
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ribonuclease H2, subunit A/RNASEH2A antibody
See all Ribonuclease H2, subunit A/RNASEH2A primary antibodies -
Description
Rabbit polyclonal to Ribonuclease H2, subunit A/RNASEH2A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab83943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 33 kDa.
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ICC |
Use a concentration of 2 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 33 kDa. |
ICC
Use a concentration of 2 - 20 µg/ml. |
Target
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Function
Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes. -
Involvement in disease
Aicardi-Goutieres syndrome 4 -
Sequence similarities
Belongs to the RNase HII family. Eukaryotic subfamily. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10535 Human
- SwissProt: O75792 Human
- Unigene: 532851 Human
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Alternative names
- AGS4 antibody
- Aicardi Goutieres syndrome 4 protein antibody
- Aicardi-Goutieres syndrome 4 protein antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab83943 has been referenced in 3 publications.
- Zhang J et al. Long noncoding RNA LINC01287 promotes proliferation and inhibits apoptosis of lung adenocarcinoma cells via the miR-3529-5p/RNASEH2A axis under the competitive endogenous RNA pattern. Environ Toxicol 36:2093-2104 (2021). PubMed: 34254728
- Riva V et al. Novel alternative ribonucleotide excision repair pathways in human cells by DDX3X and specialized DNA polymerases. Nucleic Acids Res 48:11551-11565 (2020). PubMed: 33137198
- Tan-Wong SM et al. R-Loops Promote Antisense Transcription across the Mammalian Genome. Mol Cell 76:600-616.e6 (2019). PubMed: 31679819