Overview

  • Product name

    Anti-Ribonuclease H2, subunit A/RNASEH2A antibody
    See all Ribonuclease H2, subunit A/RNASEH2A primary antibodies
  • Description

    Mouse polyclonal to Ribonuclease H2, subunit A/RNASEH2A
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Ribonuclease H2, subunit A/RNASEH2A.
    Database link: NP_006388.2

  • Positive control

    • COLO 320 HSR cell lysate, Jurkat cell lysate and HeLa cell.
  • General notes

     This product was previously labelled as Ribonuclease H2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab92876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml.
    WB: 1/500 - 1/1000. Predicted molecular weight: 33 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.Of the multiple RNases H in mammals, RNase HI is the major enzyme and shows increased activity during DNA replication. It shows more homology to the RNase HII of Escherichia coli.
    • Cellular localization

      Nuclear
    • Database links

    • Alternative names

      • AGS4 antibody
      • Aicardi Goutieres syndrome 4 protein antibody
      • EC 3.1.26.4 antibody
      • JUNB antibody
      • Ribonuclease HI large subunit antibody
      • Ribonuclease HI subunit A antibody
      • RNase H(35) antibody
      • RNase H2 subunit A antibody
      • RNase HI large subunit antibody
      • RNASEH2A antibody
      • RNASEHI antibody
      • RNHIA antibody
      • RNHL antibody
      see all

    Images

    • Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab92876) at 1/500 dilution + COLO 320 HSR at 50 µg

      Predicted band size: 33 kDa

    • Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab92876) at 1/500 dilution + Jurkat cell lysate at 50 µg

      Predicted band size: 33 kDa

    • All lanes : Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab92876) at 1/500 dilution

      Lane 1 : Ribonuclease H2, subunit A/RNASEH2A transfected 293T cell lysate
      Lane 2 : Non-transfected cell line.

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 33 kDa

    • ab92876 at 10µg/ml staining Ribonuclease H2, subunit A/RNASEH2A on Hela cell by immunofluorescence

    References

    This product has been referenced in:

    • Zimmermann M  et al. CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Nature 559:285-289 (2018). Read more (PubMed: 29973717) »
    • Kennedy EM  et al. Frequent Incorporation of Ribonucleotides during HIV-1 Reverse Transcription and Their Attenuated Repair in Macrophages. J Biol Chem 287:14280-8 (2012). WB . Read more (PubMed: 22383524) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab92876.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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