Key features and details
- Rabbit polyclonal to Ribonuclease Inhibitor/RAI
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Ribonuclease Inhibitor/RAI antibody
See all Ribonuclease Inhibitor/RAI primary antibodies
DescriptionRabbit polyclonal to Ribonuclease Inhibitor/RAI
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee
- HeLa, 293T, Jurkat, TCMK-1 and NIH3T3 whole cell lysates.
This product was previously labelled as Ribonuclease Inhibitor
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab217132 was affinity purified using an epitope specific to Ribonuclease Inhibitor/RAI immobilized on solid support.
Our Abpromise guarantee covers the use of ab217132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 50 kDa.|
FunctionRibonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play role in redox homeostasis.
Sequence similaritiesContains 15 LRR (leucine-rich) repeats.
DomainThe LRR domain forms a horseshoe-shaped structure that interacts tightly with target RNAses via a large protein interaction surface on its interior side.
modificationsThe N-terminus is blocked.
At least 30 of the 32 cysteine residues are in the reduced form.
- Information by UniProt
- AW546468 antibody
- C80305 antibody
- MGC18200 antibody
All lanes : Anti-Ribonuclease Inhibitor/RAI antibody (ab217132) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 10 seconds
Lysates were prepared using NETN lysis buffer.
Detection of Ribonuclease Inhibitor/RAI in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab217132 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab217132 has not yet been referenced specifically in any publications.