• Product name
    Anti-Ribonuclease T2 antibody
    See all Ribonuclease T2 primary antibodies
  • Description
    Rabbit polyclonal to Ribonuclease T2
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide between amino acids 13-40 from the N terminal region of Human Ribonuclease T2 (NP_003721.2).

  • Positive control
    • NCI-H460 cell line lysates; Human spleen carcinoma This antibody gave a positive result when used in the following formaldehyde fixed cell lines: LoVo



Our Abpromise guarantee covers the use of ab107313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/1000. Predicted molecular weight: 29 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Tissue specificity
    Ubiquitous. Higher expression levels observed in the temporal lobe and fetal brain.
  • Involvement in disease
    Defects in RNASET2 are the cause of leukoencephalopathy cystic without megalencephaly (LCWM) [MIM:612951]. An infantile-onset syndrome of cerebral leukoencephalopathy. Affected newborns develop microcephaly and neurologic abnormalities including psychomotor impairment, seizures and sensorineural hearing impairment. The brain shows multifocal white matter lesions, anterior temporal lobe subcortical cysts, pericystic abnormal myelination, ventriculomegaly and intracranial calcifications.
  • Sequence similarities
    Belongs to the RNase T2 family.
  • Cellular localization
    Secreted. Subcellular fractionation of transfected ovarian cancer cells reveals full-length RNASET2 in the endoplasmic reticulum fraction and the 2 smaller RNASET2 proteolytic products in the lysosome fraction.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA514O12.3 antibody
    • Ribonuclease 6 antibody
    • Ribonuclease T2 antibody
    • RNASE6PL antibody
    • Rnaset2 antibody
    • RNT2_HUMAN antibody
    • RP11 514O12.3 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen carcinoma tissue sections labeling Ribonuclease T2 with ab107313 at 1/25 dilution. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Anti-Ribonuclease T2 antibody (ab107313) at 1/1000 dilution + NCI-H460 whole cell lysate at 35 µg

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 29 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • ab107313 stained LoVo cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107313 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Wang Q  et al. Stress-induced RNASET2 overexpression mediates melanocyte apoptosis via the TRAF2 pathway in vitro. Cell Death Dis 5:e1022 (2014). WB ; Human . Read more (PubMed: 24457966) »

See 1 Publication for this product

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