Overview

  • Product name

    Anti-Ribonuclease U2 antibody
  • Description

    Rabbit polyclonal to Ribonuclease U2
  • Host species

    Rabbit
  • Specificity

    Predicted to react with Ustilago sphaerogena.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Recombinant full length protein corresponding to Ribonuclease U2 aa 1-114. (Ustilago sphaerogena). (With N-terminal SUMO tag).
    Sequence:

    CDIPQSTNCGGNVYSNDDINTAIQGALDDVANGDRPDNYPHQYYDEASED ITLCCGSGPWSEFPLVYNGPYYSSRDNYVSPGPDRVIYQTNTGEFCATVT HTGAASYDGFTQCS


    Database link: P00654

  • Positive control

    • WB: Recombinant Ustilago sphaerogena Ribonuclease U2 protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab234677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 12 kDa).

Images

  • All lanes : Anti-Ribonuclease U2 antibody (ab234677) at 1/500 dilution

    Lane 1 : Recombinant Ustilago sphaerogena Ribonuclease U2 protein, 80 ng
    Lane 2 : Recombinant Ustilago sphaerogena Ribonuclease U2 protein, 40 ng
    Lane 3 : Recombinant Ustilago sphaerogena Ribonuclease U2 protein, 20 ng
    Lane 4 : Recombinant Ustilago sphaerogena Ribonuclease U2 protein, 10 ng

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 12 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    Predicted MW of recombinant protein with N-terminal SUMO tag: 28 kDa.

References

ab234677 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab234677.
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