Overview

  • Product name
    Anti-Ribophorin I antibody
    See all Ribophorin I primary antibodies
  • Description
    Rabbit polyclonal to Ribophorin I
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH (10-30 aa in length) at the C-term of last 50 aa of human ribophorin 1.

  • Positive control
    • HeLa cell lysate. Mouse liver tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column, eluted out with both high and low pH buffers, neutralized immediately after elution then followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 69 kDa.

Target

  • Function
    Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
  • Tissue specificity
    Expressed in all tissues tested.
  • Sequence similarities
    Belongs to the OST1 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • D6Wsu137e antibody
    • Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit antibody
    • Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit precursor antibody
    • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit antibody
    • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 antibody
    • EC 2.4.1.119 antibody
    • Oligosaccharyltransferase 1 homolog antibody
    • oligosaccharyltransferase complex subunit (non-catalytic) antibody
    • OST1 antibody
    • RBPH1 antibody
    • Ribophorin I antibody
    • Ribophorin-1 antibody
    • RPN-I antibody
    • Rpn1 antibody
    • RPN1_HUMAN antibody
    see all

Images

  • All lanes : Anti-Ribophorin I antibody (ab38451) at 1/100 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Mouse liver tissue lysate

    Predicted band size: 69 kDa
    Observed band size: 69 kDa
    Additional bands at: 32 kDa, 50 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Akizu N  et al. Biallelic mutations in SNX14 cause a syndromic form of cerebellar atrophy and lysosome-autophagosome dysfunction. Nat Genet 47:528-34 (2015). Read more (PubMed: 25848753) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Ok, sorry for the missunderstanding. If you are interested in the testing discount in the future please do not hesitate to let us know. We are also currenlty running a promotion whereby if you think you are able to provide a new image for an antibody (i.e a WB or IHC image) for an antibody which does not currently have such an image on the datasheet then you would also qualify for a testing discount. If you would like more details of this offer please let me know.

Read More

Answer

Thank you for contacting us. We offer testing discount for applications and species that have not been tested with the antibody previously. For example for this antibody if you were to use if for a species other than human or mouse, or for another application such as IHC or flow cytometry. If this were to be the case, we could issue a testing disount code. You would then purchase the antibody (at full price), test the antibody for new application or species and share the results with us through an abreview, stating the discount code issued in the additional notes field (within four months of issue). Once this has been completed you would be eligable for a free primary antibody of your choice. More information on this dicount can be found here: www.abcam.com/collaborationdiscount and information on the Abreview system can be found here: www.abcam.com/abreviews It does not matter if the result is positive or negative as long as you share it with us through the Abreview system you will be eligable for the discount code. If you are interested in this offer please specify which species and application you wish to use the antibody for and I can provide you with a discount code. If you have any further questions please do not hesistate to ask.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up