RecombinantRabMAb

Recombinant Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17043(B)] to Ribophorin I - N-terminal
  • Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal
    See all Ribophorin I primary antibodies

  • Description

    Rabbit monoclonal [EPR17043(B)] to Ribophorin I - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Ribophorin I aa 1-100. The exact sequence is proprietary.
    Database link: P04843

  • Positive control

    • WB: BxPC-3, HepG2, HeLa and PC-3 cell lysates; Human fetal liver lysate. IHC-P: Human placenta and rat liver tissues. ICC/IF: HeLa and MCF7 cells Flow Cyt: HeLa cell

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17043(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab198508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
Flow Cyt 1/100.

Target

  • Function

    Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.

  • Tissue specificity

    Expressed in all tissues tested.

  • Sequence similarities

    Belongs to the OST1 family.

  • Cellular localization

    Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    • Alternative names

      • D6Wsu137e antibody
      • Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit antibody
      • Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit precursor antibody
      • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit antibody
      • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 antibody
      • EC 2.4.1.119 antibody
      • Oligosaccharyltransferase 1 homolog antibody
      • oligosaccharyltransferase complex subunit (non-catalytic) antibody
      • OST1 antibody
      • RBPH1 antibody
      • Ribophorin I antibody
      • Ribophorin-1 antibody
      • RPN-I antibody
      • Rpn1 antibody
      • RPN1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Western blot - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      All lanes : Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508) at 1/5000 dilution

      Lane 1 : BxPC-3 (Human pancreas adenocarcinoma cells) cell lysate
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
      Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
      Lane 4 : PC-3 (Human prostate cancer cell line) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 69 kDa
      Observed band size: 69 kDa


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

      Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat liver tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

      Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab198508 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    • Flow Cytometry - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Flow Cytometry - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

      Flow cytometry analysis of HeLa cells labelling Ribophorin I (red) with purified ab198508 at dilution of 1/100. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

    • Western blot - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Western blot - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508) at 1/2000 dilution + Human fetal liver lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 69 kDa
      Observed band size: 69 kDa


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human placenta tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

      Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab198508 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    • Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

    References (2)

    Publishing research using ab198508? Please let us know so that we can cite the reference in this datasheet.

    ab198508 has been referenced in 2 publications.

    • Millership SJ  et al. Neuronatin regulates pancreatic ß cell insulin content and secretion. J Clin Invest 128:3369-3381 (2018). PubMed: 29864031
    • Yin P  et al. Sec24C-Dependent Transport of Claudin-1 Regulates Hepatitis C Virus Entry. J Virol 91:N/A (2017). PubMed: 28679754

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