Recombinant Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17043(B)] to Ribophorin I - N-terminal
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal
See all Ribophorin I primary antibodies -
Description
Rabbit monoclonal [EPR17043(B)] to Ribophorin I - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: BxPC-3, HepG2, HeLa and PC-3 cell lysates; Human fetal liver lysate. IHC-P: Human placenta and rat liver tissues. ICC/IF: HeLa and MCF7 cells Flow Cyt (intra): HeLa cell
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17043(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab198508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100.
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ICC/IF |
1/250.
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/2000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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Notes |
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Flow Cyt (Intra)
1/100. |
ICC/IF
1/250. |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/2000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
Target
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Function
Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. -
Tissue specificity
Expressed in all tissues tested. -
Sequence similarities
Belongs to the OST1 family. -
Cellular localization
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 6184 Human
- Entrez Gene: 25596 Rat
- Omim: 180470 Human
- SwissProt: P04843 Human
- SwissProt: P07153 Rat
- Unigene: 518244 Human
- Unigene: 603636 Human
- Unigene: 4224 Rat
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Alternative names
- D6Wsu137e antibody
- Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit antibody
- Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit precursor antibody
see all
Images
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All lanes : Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508) at 1/5000 dilution
Lane 1 : BxPC-3 (Human pancreas adenocarcinoma cells) cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lane 4 : PC-3 (Human prostate cancer cell line) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab198508 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
Intracellular Flow Cytometry analysis of HeLa cells labelling Ribophorin I (red) with purified ab198508 at dilution of 1/100. The secondary antibody used was Alexa Fluorr® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
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Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508) at 1/2000 dilution + Human fetal liver lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human placenta tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab198508 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (4)
ab198508 has been referenced in 4 publications.
- Tomida S et al. The SH3 domain in the fucosyltransferase FUT8 controls FUT8 activity and localization and is essential for core fucosylation. J Biol Chem 295:7992-8004 (2020). PubMed: 32350116
- Knopf JD et al. Intramembrane protease RHBDL4 cleaves oligosaccharyltransferase subunits to target them for ER-associated degradation. J Cell Sci 133:N/A (2020). PubMed: 32005703
- Millership SJ et al. Neuronatin regulates pancreatic ß cell insulin content and secretion. J Clin Invest 128:3369-3381 (2018). PubMed: 29864031
- Yin P et al. Sec24C-Dependent Transport of Claudin-1 Regulates Hepatitis C Virus Entry. J Virol 91:N/A (2017). PubMed: 28679754