Recombinant
RabMAb

Recombinant Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

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Overview

  • Product name

    Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal
    See all Ribophorin I primary antibodies

  • Description

    Rabbit monoclonal [EPR17043(B)] to Ribophorin I - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Ribophorin I aa 1-100. The exact sequence is proprietary.
    Database link: P04843

  • Positive control

    • WB: BxPC-3, HepG2, HeLa and PC-3 cell lysates; Human fetal liver lysate. IHC-P: Human placenta and rat liver tissues. ICC/IF: HeLa and MCF7 cells FC: HeLa cell

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17043(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab198508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
Flow Cyt 1/100.

Target

  • Function

    Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.

  • Tissue specificity

    Expressed in all tissues tested.

  • Sequence similarities

    Belongs to the OST1 family.

  • Cellular localization

    Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    • Alternative names

      • D6Wsu137e antibody
      • Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit antibody
      • Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit precursor antibody
      • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit antibody
      • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 antibody
      • EC 2.4.1.119 antibody
      • Oligosaccharyltransferase 1 homolog antibody
      • oligosaccharyltransferase complex subunit (non-catalytic) antibody
      • OST1 antibody
      • RBPH1 antibody
      • Ribophorin I antibody
      • Ribophorin-1 antibody
      • RPN-I antibody
      • Rpn1 antibody
      • RPN1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Western blot - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      All lanes : Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508) at 1/5000 dilution

      Lane 1 : BxPC-3 (Human pancreas adenocarcinoma cells) cell lysate
      Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
      Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
      Lane 4 : PC-3 (Human prostate cancer cell line) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 69 kDa
      Observed band size: 69 kDa


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

      Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat liver tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

      Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab198508 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    • Flow Cytometry - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Flow Cytometry - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

      Flow cytometry analysis of HeLa cells labelling Ribophorin I (red) with purified ab198508 at dilution of 1/100. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

    • Western blot - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Western blot - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508) at 1/2000 dilution + Human fetal liver lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 69 kDa
      Observed band size: 69 kDa


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human placenta tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)
      Immunocytochemistry/ Immunofluorescence - Anti-Ribophorin I antibody [EPR17043(B)] - N-terminal (ab198508)

      Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Ribophorin I with ab198508 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab198508 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    References

    This product has been referenced in:

    See all 2 Publications for this product

    Publishing research using ab198508? Please let us know so that we can cite the reference in this datasheet.

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