Overview

  • Product name

    Anti-Ribophorin I antibody [EPR17044(B)]
    See all Ribophorin I primary antibodies
  • Description

    Rabbit monoclonal [EPR17044(B)] to Ribophorin I
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Ribophorin I aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: P04843

  • Positive control

    • WB: HeLa, BxPC-3, C6, and PC-12 whole cell lysates. Rat kidney and spleen lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17044(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 69 kDa.

Target

  • Function

    Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
  • Tissue specificity

    Expressed in all tissues tested.
  • Sequence similarities

    Belongs to the OST1 family.
  • Cellular localization

    Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • D6Wsu137e antibody
    • Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit antibody
    • Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit precursor antibody
    • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit antibody
    • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 antibody
    • EC 2.4.1.119 antibody
    • Oligosaccharyltransferase 1 homolog antibody
    • oligosaccharyltransferase complex subunit (non-catalytic) antibody
    • OST1 antibody
    • RBPH1 antibody
    • Ribophorin I antibody
    • Ribophorin-1 antibody
    • RPN-I antibody
    • Rpn1 antibody
    • RPN1_HUMAN antibody
    see all

Images

  • All lanes : Anti-Ribophorin I antibody [EPR17044(B)] (ab197888) at 1/20000 dilution

    Lane 1 : BxPC-3 (human Pancreas adenocarcinoma ) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 69 kDa


    Exposure time: 1 minute


    Blocking and diluting buffer: 5% NFDM/TBST

  • All lanes : Anti-Ribophorin I antibody [EPR17044(B)] (ab197888) at 1/2000 dilution

    Lane 1 : Rat kidney lysate
    Lane 2 : Rat spleen lysate
    Lane 3 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 69 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST

References

ab197888 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab197888.
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