Overview

  • Product name
    Anti-Ribosomal protein S10 antibody [EPR8545]
    See all Ribosomal protein S10 primary antibodies
  • Description
    Rabbit monoclonal [EPR8545] to Ribosomal protein S10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ribosomal protein S10 aa 100-200. The exact sequence is proprietary.

  • Positive control
    • WB: HeLa, K562, Raji, C6, Raw264.7, PC-12 and NIH/3T3 cell lysates; IHC-P: Human kidney tissue, Human gastric carcinoma, Human pancreas, Mouse stomach, Rat stomach; ICC: HeLa cells. FC: HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR8545
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1000.
ICC/IF 1/250 - 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
IHC-P 1/50 - 1/100.

See IHC antigen retrieval protocols.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Component of the 40S ribosomal subunit.
    • Involvement in disease
      Defects in RPS10 are the cause of Diamond-Blackfan anemia type 9 (DBA9) [MIM:613308]. It is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre- Robin syndrome and cleft palate), thumb and urogenital anomalies.
    • Sequence similarities
      Belongs to the ribosomal protein S10e family.
    • Post-translational
      modifications
      Methylated by PRMT5. Methylation is necessary for its interaction with NPS1, its localization in the granular component (GC) region of the nucleolus, for the proper assembly of ribosomes, protein synthesis and optimal cell proliferation.
    • Cellular localization
      Cytoplasm. Nucleus > nucleolus. Localized in the granular component (GC) region of the nucleolus. Methylation is required for its localization in the GC region. Co-localizes with NPS1 in the GC region of the nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 40S ribosomal protein S10 antibody
      • DBA9 antibody
      • MGC88819 antibody
      • OTTHUMP00000016229 antibody
      • OTTHUMP00000016230 antibody
      • RPS10 antibody
      • RS10_HUMAN antibody
      • S10 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling Ribosomal protein S10 with Purified ab151550 at 1:100 dilution (11.86 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ribosomal protein S10 with Purified ab151550 at 1:1000 dilution (1 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ribosomal protein S10 with Purified ab151550 at 1:250 dilution (4.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling Ribosomal protein S10 with Purified ab151550 at 1:100 dilution (11.86 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas tissue sections labeling Ribosomal protein S10 with Purified ab151550 at 1:100 dilution (11.86 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling Ribosomal protein S10 with Purified ab151550 at 1:100 dilution (11.86 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • All lanes : Anti-Ribosomal protein S10 antibody [EPR8545] (ab151550) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : C6 cell lysate
      Lane 5 : Raw264.7 cell lysate
      Lane 6 : PC12 cell lysate
      Lane 7 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 19 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ribosomal protein S10 with ab151550 at 1/50 dilution.
    • Immunofluorescent analysis of HeLa cells labelling Ribosomal protein S10 with ab151550 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151550 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab151550 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151550 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab151550 showing +ve staining.

    References

    This product has been referenced in:
    See all 8 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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