Recombinant
RabMAb

Anti-Ribosomal protein S10 antibody [EPR8545] (ab151550)

Overview

  • Product name
    Anti-Ribosomal protein S10 antibody [EPR8545]
  • Description
    Rabbit monoclonal [EPR8545] to Ribosomal protein S10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ribosomal protein S10 aa 100-200. The exact sequence is proprietary.

  • Positive control
    • HeLa, K562, Raji, C6, Raw264.7, PC-12 and NIH/3T3 cell lysates; Human kidney tissue; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR8545
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
IHC-P 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Component of the 40S ribosomal subunit.
    • Involvement in disease
      Defects in RPS10 are the cause of Diamond-Blackfan anemia type 9 (DBA9) [MIM:613308]. It is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre- Robin syndrome and cleft palate), thumb and urogenital anomalies.
    • Sequence similarities
      Belongs to the ribosomal protein S10e family.
    • Post-translational
      modifications
      Methylated by PRMT5. Methylation is necessary for its interaction with NPS1, its localization in the granular component (GC) region of the nucleolus, for the proper assembly of ribosomes, protein synthesis and optimal cell proliferation.
    • Cellular localization
      Cytoplasm. Nucleus > nucleolus. Localized in the granular component (GC) region of the nucleolus. Methylation is required for its localization in the GC region. Co-localizes with NPS1 in the GC region of the nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 40S ribosomal protein S10 antibody
      • DBA9 antibody
      • MGC88819 antibody
      • OTTHUMP00000016229 antibody
      • OTTHUMP00000016230 antibody
      • RPS10 antibody
      • RS10_HUMAN antibody
      • S10 antibody
      see all

    Images

    • All lanes : Anti-Ribosomal protein S10 antibody [EPR8545] (ab151550) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : C6 cell lysate
      Lane 5 : Raw264.7 cell lysate
      Lane 6 : PC12 cell lysate
      Lane 7 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 19 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ribosomal protein S10 with ab151550 at 1/50 dilution.
    • Immunofluorescent analysis of HeLa cells labelling Ribosomal protein S10 with ab151550 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151550 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab151550 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151550 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab151550 showing +ve staining.

    References

    This product has been referenced in:
    • Juszkiewicz S & Hegde RS Initiation of Quality Control during Poly(A) Translation Requires Site-Specific Ribosome Ubiquitination. Mol Cell 65:743-750.e4 (2017). Read more (PubMed: 28065601) »
    • Larburu N  et al. Structure of a human pre-40S particle points to a role for RACK1 in the final steps of 18S rRNA processing. Nucleic Acids Res 44:8465-78 (2016). WB . Read more (PubMed: 27530427) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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