Recombinant Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11487] to Ribosomal protein S11/RPS11
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Ribosomal protein S11/RPS11 antibody [EPR11487]
See all Ribosomal protein S11/RPS11 primary antibodies -
Description
Rabbit monoclonal [EPR11487] to Ribosomal protein S11/RPS11 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Ribosomal protein S11/RPS11 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P62280 -
Positive control
- Jurkat, MCF7, HeLa and 293T cell lysates; Human thyroid carcinoma and uterus tisssue; MCF7 and HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11487 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 18 kDa.
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ICC/IF |
1/100 - 1/250.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 18 kDa. |
ICC/IF
1/100 - 1/250. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the ribosomal protein S17P family. - Information by UniProt
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Database links
- Entrez Gene: 6205 Human
- Entrez Gene: 27207 Mouse
- Entrez Gene: 81774 Rat
- Omim: 180471 Human
- SwissProt: P62280 Human
- SwissProt: P62281 Mouse
- SwissProt: P62282 Rat
- Unigene: 433529 Human
see all -
Alternative names
- 40S ribosomal protein S11 antibody
- RPS 11 antibody
- rps11 antibody
see all
Images
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Immunofluorescent staining of MCF7 cells labeling Ribosomal protein S11/RPS11 with ab175213 at 1/100 dilution (red). DAPI nuclear staining (blue).
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All lanes : Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 18 kDa -
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling Ribosomal protein S11/RPS11 with ab175213 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling Ribosomal protein S11/RPS11 with ab175213 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of permeabilized HeLacells labeling Ribosomal protein S11/RPS11 with ab175213at 1/10 dilution (red) compared toa Rabbit IgGnegative control (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab175213 has been referenced in 4 publications.
- Zhou C et al. High RPS11 level in hepatocellular carcinoma associates with poor prognosis after curative resection. Ann Transl Med 8:466 (2020). PubMed: 32395510
- McHugh CA & Guttman M RAP-MS: A Method to Identify Proteins that Interact Directly with a Specific RNA Molecule in Cells. Methods Mol Biol 1649:473-488 (2018). PubMed: 29130217
- Valvezan AJ et al. mTORC1 Couples Nucleotide Synthesis to Nucleotide Demand Resulting in a Targetable Metabolic Vulnerability. Cancer Cell 32:624-638.e5 (2017). PubMed: 29056426
- Marshall DJ et al. Extracellular Antibody Drug Conjugates Exploiting the Proximity of Two Proteins. Mol Ther N/A:N/A (2016). WB . PubMed: 27434591