Recombinant Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11487] to Ribosomal protein S11/RPS11
- Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Ribosomal protein S11/RPS11 antibody [EPR11487]
See all Ribosomal protein S11/RPS11 primary antibodies -
Description
Rabbit monoclonal [EPR11487] to Ribosomal protein S11/RPS11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Ribosomal protein S11/RPS11 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P62280 -
Positive control
- Jurkat, MCF7, HeLa and 293T cell lysates; Human thyroid carcinoma and uterus tisssue; MCF7 and HeLa cells.
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General notes
This product was previously labelled as Ribosomal protein S11
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11487 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab175213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/5000. Predicted molecular weight: 18 kDa. | |
ICC/IF | 1/100 - 1/250. | |
Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the ribosomal protein S17P family. - Information by UniProt
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Database links
- Entrez Gene: 6205 Human
- Entrez Gene: 27207 Mouse
- Entrez Gene: 81774 Rat
- Omim: 180471 Human
- SwissProt: P62280 Human
- SwissProt: P62281 Mouse
- SwissProt: P62282 Rat
- Unigene: 433529 Human
see all -
Alternative names
- 40S ribosomal protein S11 antibody
- RPS 11 antibody
- rps11 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
Immunofluorescent staining of MCF7 cells labeling Ribosomal protein S11/RPS11 with ab175213 at 1/100 dilution (red). DAPI nuclear staining (blue).
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All lanes : Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 18 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling Ribosomal protein S11/RPS11 with ab175213 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling Ribosomal protein S11/RPS11 with ab175213 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow cytometric analysis of permeabilized HeLa cells labeling Ribosomal protein S11/RPS11 with ab175213 at 1/10 dilution (red) compared to a Rabbit IgG negative control (green).
Protocols
Datasheets and documents
References (3)
ab175213 has been referenced in 3 publications.
- Zhou C et al. High RPS11 level in hepatocellular carcinoma associates with poor prognosis after curative resection. Ann Transl Med 8:466 (2020). PubMed: 32395510
- Valvezan AJ et al. mTORC1 Couples Nucleotide Synthesis to Nucleotide Demand Resulting in a Targetable Metabolic Vulnerability. Cancer Cell 32:624-638.e5 (2017). PubMed: 29056426
- Marshall DJ et al. Extracellular Antibody Drug Conjugates Exploiting the Proximity of Two Proteins. Mol Ther N/A:N/A (2016). WB . PubMed: 27434591