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    ribosomal-protein-s11rps11-antibody-epr11487-ab175213.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome
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RecombinantRabMAb

Recombinant Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
  • Western blot - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
  • Flow Cytometry - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
  • Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11487] to Ribosomal protein S11/RPS11
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Ribosomal protein S11/RPS11 antibody [EPR11487]
    See all Ribosomal protein S11/RPS11 primary antibodies
  • Description

    Rabbit monoclonal [EPR11487] to Ribosomal protein S11/RPS11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ribosomal protein S11/RPS11 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P62280

  • Positive control

    • Jurkat, MCF7, HeLa and 293T cell lysates; Human thyroid carcinoma and uterus tisssue; MCF7 and HeLa cells.
  • General notes

     

     

     This product was previously labelled as Ribosomal protein S11

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11487
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome

Associated products

  • Alternative Versions

    • Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)

Applications

Our Abpromise guarantee covers the use of ab175213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 18 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Sequence similarities

      Belongs to the ribosomal protein S17P family.
    • Target information above from: UniProt accession P62280 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6205 Human
      • Entrez Gene: 27207 Mouse
      • Entrez Gene: 81774 Rat
      • Omim: 180471 Human
      • SwissProt: P62280 Human
      • SwissProt: P62281 Mouse
      • SwissProt: P62282 Rat
      • Unigene: 433529 Human
      • Unigene: 196538 Mouse
      • Unigene: 389435 Mouse
      • Unigene: 2177 Rat
      see all
    • Alternative names

      • 40S ribosomal protein S11 antibody
      • RPS 11 antibody
      • rps11 antibody
      • RS11_HUMAN antibody
      • S11 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
      Immunocytochemistry/ Immunofluorescence - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)

      Immunofluorescent staining of MCF7 cells labeling Ribosomal protein S11/RPS11 with ab175213 at 1/100 dilution (red). DAPI nuclear staining (blue).

    • Western blot - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
      Western blot - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
      All lanes : Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 18 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)

      Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling Ribosomal protein S11/RPS11 with ab175213 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)

      Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling Ribosomal protein S11/RPS11 with ab175213 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
      Flow Cytometry - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)

      Flow cytometric analysis of permeabilized HeLa cells labeling Ribosomal protein S11/RPS11 with ab175213 at 1/10 dilution (red) compared to a Rabbit IgG negative control (green).

    • Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
      Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab175213? Please let us know so that we can cite the reference in this datasheet.

    ab175213 has been referenced in 3 publications.

    • Zhou C  et al. High RPS11 level in hepatocellular carcinoma associates with poor prognosis after curative resection. Ann Transl Med 8:466 (2020). PubMed: 32395510
    • Valvezan AJ  et al. mTORC1 Couples Nucleotide Synthesis to Nucleotide Demand Resulting in a Targetable Metabolic Vulnerability. Cancer Cell 32:624-638.e5 (2017). PubMed: 29056426
    • Marshall DJ  et al. Extracellular Antibody Drug Conjugates Exploiting the Proximity of Two Proteins. Mol Ther N/A:N/A (2016). WB . PubMed: 27434591

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab175213.
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