Recombinant
RabMAb

Recombinant Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)

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Overview

  • Product name

    Anti-Ribosomal protein S11/RPS11 antibody [EPR11487]
    See all Ribosomal protein S11/RPS11 primary antibodies

  • Description

    Rabbit monoclonal [EPR11487] to Ribosomal protein S11/RPS11

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Ribosomal protein S11/RPS11 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P62280

  • Positive control

    • Jurkat, MCF7, HeLa and 293T cell lysates; Human thyroid carcinoma and uterus tisssue; MCF7 and HeLa cells.

  • General notes

     

     

     This product was previously labelled as Ribosomal protein S11

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11487

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 18 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.

    • Target

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
      Immunocytochemistry/ Immunofluorescence - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)

      Immunofluorescent staining of MCF7 cells labeling Ribosomal protein S11/RPS11 with ab175213 at 1/100 dilution (red). DAPI nuclear staining (blue).

    • Western blot - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
      Western blot - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
      All lanes : Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 18 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)

      Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling Ribosomal protein S11/RPS11 with ab175213 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)

      Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling Ribosomal protein S11/RPS11 with ab175213 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)
      Flow Cytometry - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] (ab175213)

      Flow cytometric analysis of permeabilized HeLa cells labeling Ribosomal protein S11/RPS11 with ab175213 at 1/10 dilution (red) compared to a Rabbit IgG negative control (green).

    References

    This product has been referenced in:

    • Valvezan AJ  et al. mTORC1 Couples Nucleotide Synthesis to Nucleotide Demand Resulting in a Targetable Metabolic Vulnerability. Cancer Cell 32:624-638.e5 (2017). Read more (PubMed: 29056426) »
    • Marshall DJ  et al. Extracellular Antibody Drug Conjugates Exploiting the Proximity of Two Proteins. Mol Ther N/A:N/A (2016). WB . Read more (PubMed: 27434591) »
    See all 2 Publications for this product

    Publishing research using ab175213? Please let us know so that we can cite the reference in this datasheet.

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