RecombinantRabMAb

Recombinant Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)

Submit a review Submit a question
Immunocytochemistry/ Immunofluorescence - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)
  • Flow Cytometry - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)
  • Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11487] to Ribosomal protein S11/RPS11 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free
    See all Ribosomal protein S11/RPS11 primary antibodies

  • Description

    Rabbit monoclonal [EPR11487] to Ribosomal protein S11/RPS11 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab240183 is the carrier-free version of ab175213 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240183 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Ribosomal protein S11

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11487

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab240183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.
Notes
Flow Cyt
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.
  • Application notes
    Is unsuitable for IP.

    • Target

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)
      Immunocytochemistry/ Immunofluorescence - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)

      Immunofluorescent staining of MCF7 cells labeling Ribosomal protein S11/RPS11 with ab175213 at 1/100 dilution (red). DAPI nuclear staining (blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175213).

    • Flow Cytometry - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)
      Flow Cytometry - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)

      Flow cytometric analysis of permeabilized HeLa cells labeling Ribosomal protein S11/RPS11 with ab175213 at 1/10 dilution (red) compared to a Rabbit IgG negative control (green).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175213).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)

      Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling Ribosomal protein S11/RPS11 with ab175213 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175213).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)

      Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling Ribosomal protein S11/RPS11 with ab175213 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175213).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)
      Anti-Ribosomal protein S11/RPS11 antibody [EPR11487] - BSA and Azide free (ab240183)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab240183? Please let us know so that we can cite the reference in this datasheet.

    ab240183 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab240183.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com