The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
The inhibition of protein synthesis by specific anti 5.8S rRNA oligonucleotides have suggested that this RNA plays an important role in eukaryotic ribosome function, specifically ribosomal translocation. Mutations in the 5.8S rRNA can inhibit cell growth and compromise protein synthesis in vitro.
Immunofluorescence analysis of formaldehyde-fixed HeLa cells labeling ribosomal RNA 5.8s (green) using ab171119 at a 1/25 dilution. Cells were washed with PBS and incubated with goat anti-rabbit IgG (H+L) secondary antibody, DyLight 488 conjugated. Nuclei (blue) were stained with Hoechst 33342 dye.