Overview

  • Product name

    Anti-ribosomal RNA antibody [Y10B]
  • Description

    Mouse monoclonal [Y10B] to ribosomal RNA
  • Host species

    Mouse
  • Specificity

    This clone may also detect large (28S) and small (18S) rRNA (PMID:7496633).
  • Tested applications

    Suitable for: IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Other Immunogen Type corresponding to ribosomal RNA 5.8s.

  • Positive control

    • HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    Y10B
  • Isotype

    IgG3
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF 1/25.

Target

  • Relevance

    The inhibition of protein synthesis by specific anti 5.8S rRNA oligonucleotides have suggested that this RNA plays an important role in eukaryotic ribosome function, specifically ribosomal translocation. Mutations in the 5.8S rRNA can inhibit cell growth and compromise protein synthesis in vitro.
  • Cellular localization

    Cytoplasmic and Nuclear

Images

  • Immunofluorescence analysis of formaldehyde-fixed HeLa cells labeling ribosomal RNA 5.8s (green) using ab171119 at a 1/25 dilution. Cells were washed with PBS and incubated with goat anti-rabbit IgG (H+L) secondary antibody, DyLight 488 conjugated. Nuclei (blue) were stained with Hoechst 33342 dye.

References

This product has been referenced in:

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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