Key features and details
- Mouse monoclonal [Y10B] to ribosomal RNA
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG3
Product nameAnti-ribosomal RNA antibody [Y10B]
DescriptionMouse monoclonal [Y10B] to ribosomal RNA
SpecificityThis clone may also detect large (28S) and small (18S) rRNA (PMID:7496633).
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: a wide range of other species
Tissue, cells or virus corresponding to ribosomal RNA. Whole ribosomal rRNA 5.8S
- HeLa cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Light chain typekappa
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceThe inhibition of protein synthesis by specific anti 5.8S rRNA oligonucleotides have suggested that this RNA plays an important role in eukaryotic ribosome function, specifically ribosomal translocation. Mutations in the 5.8S rRNA can inhibit cell growth and compromise protein synthesis in vitro.
Cellular localizationCytoplasmic and Nuclear
ab171119 has been referenced in 6 publications.
- Alves MJ et al. CCNA2 Ablation in Aged Mice Results in Abnormal rRNA Granule Accumulation in Hippocampus. Am J Pathol 189:426-439 (2019). PubMed: 30579783
- Shigeoka T et al. On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons. Cell Rep 29:3605-3619.e10 (2019). PubMed: 31825839
- Chau KF et al. Downregulation of ribosome biogenesis during early forebrain development. Elife 7:N/A (2018). PubMed: 29745900
- Kershner L & Welshhans K RACK1 is necessary for the formation of point contacts and regulates axon growth. Dev Neurobiol 77:1038-1056 (2017). PubMed: 28245531
- Kalinski AL et al. mRNAs and Protein Synthetic Machinery Localize into Regenerating Spinal Cord Axons When They Are Provided a Substrate That Supports Growth. J Neurosci 35:10357-70 (2015). PubMed: 26180210
- Spillane M et al. Mitochondria coordinate sites of axon branching through localized intra-axonal protein synthesis. Cell Rep 5:1564-75 (2013). PubMed: 24332852