Overview

  • Product name
    Anti-ribosomal RNA antibody [Y10B]
  • Description
    Mouse monoclonal [Y10B] to ribosomal RNA
  • Host species
    Mouse
  • Specificity
    This clone may also detect large (28S) and small (18S) rRNA (PMID:7496633).
  • Tested applications
    Suitable for: IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Other Immunogen Type corresponding to ribosomal RNA 5.8s.

  • Positive control
    • HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    Y10B
  • Isotype
    IgG3
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF 1/25.

Target

  • Relevance
    The inhibition of protein synthesis by specific anti 5.8S rRNA oligonucleotides have suggested that this RNA plays an important role in eukaryotic ribosome function, specifically ribosomal translocation. Mutations in the 5.8S rRNA can inhibit cell growth and compromise protein synthesis in vitro.
  • Cellular localization
    Cytoplasmic and Nuclear

Images

  • Immunofluorescence analysis of formaldehyde-fixed HeLa cells labeling ribosomal RNA 5.8s (green) using ab171119 at a 1/25 dilution. Cells were washed with PBS and incubated with goat anti-rabbit IgG (H+L) secondary antibody, DyLight 488 conjugated. Nuclei (blue) were stained with Hoechst 33342 dye.

References

This product has been referenced in:
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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