Anti-RIC8A antibody (ab97808)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-RIC8A antibody
See all RIC8A primary antibodies -
Description
Rabbit polyclonal to RIC8A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region within amino acids 528 and 537 of Human RIC8A (NP_068751).
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Positive control
- A431, H1299, HeLa, HepG2 and Raji cell lines; Adrenal cortical tumor
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab97808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 24466196 | |
| IP | Use at an assay dependent concentration. PubMed: 24466196 | |
| IHC-P | 1/100 - 1/500. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 60 kDa. |
Target
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Function
Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation. -
Sequence similarities
Belongs to the synembryn family. -
Cellular localization
Cytoplasm. Cell membrane. Colocalizes with RIC8A in CA2 hippocampal neurons. Colocalizes with GNAI1 and RGS14 at the plasma membrane (By similarity). - Information by UniProt
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Database links
- Entrez Gene: 60626 Human
- Omim: 609146 Human
- SwissProt: Q9NPQ8 Human
- Unigene: 592292 Human
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Alternative names
- MGC104517 antibody
- MGC131931 antibody
- MGC148073 antibody
see all
Images
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Western blot - Anti-RIC8A antibody (ab97808)All lanes : Anti-RIC8A antibody (ab97808) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 60 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIC8A antibody (ab97808)ab97808, at a 1/100 dilution, staining RIC8A in paraffin embedded Adrenal cortical tumor by Immunohistochemistry.
Protocols
References
This product has been referenced in:
- Chishiki K et al. Ric-8A, an activator protein of Gai, controls mammalian epithelial cell polarity for tight junction assembly and cystogenesis. Genes Cells 22:293-309 (2017). Read more (PubMed: 28185378) »
- Boularan C et al. Resistance to inhibitors of cholinesterase (Ric)-8A and Gai contribute to cytokinesis abscission by controlling vacuolar protein-sorting (Vps)34 activity. PLoS One 9:e86680 (2014). WB, IP, ICC/IF ; Human . Read more (PubMed: 24466196) »
