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    ric8a-antibody-ab97808.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Heterotrimeric G Proteins Regulators
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Anti-RIC8A antibody (ab97808)

  • Datasheet
  • SDS
Submit a review Submit a question References (3)

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Western blot - Anti-RIC8A antibody (ab97808)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIC8A antibody (ab97808)

Key features and details

  • Rabbit polyclonal to RIC8A
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-RIC8A antibody
    See all RIC8A primary antibodies
  • Description

    Rabbit polyclonal to RIC8A
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 528 and 537 of Human RIC8A (NP_068751).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Heterotrimeric G Proteins
    • Regulators

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Hep G2 cytoplasmic extract lysate (ab14659)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab7909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
IHC-P
1/100 - 1/500.
WB
1/500 - 1/3000. Predicted molecular weight: 60 kDa.
Notes
IHC-P
1/100 - 1/500.
WB
1/500 - 1/3000. Predicted molecular weight: 60 kDa.

Target

  • Function

    Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.
  • Sequence similarities

    Belongs to the synembryn family.
  • Cellular localization

    Cytoplasm. Cell membrane. Colocalizes with RIC8A in CA2 hippocampal neurons. Colocalizes with GNAI1 and RGS14 at the plasma membrane (By similarity).
  • Target information above from: UniProt accession Q9NPQ8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 60626 Human
    • Omim: 609146 Human
    • SwissProt: Q9NPQ8 Human
    • Unigene: 592292 Human
    • Alternative names

      • MGC104517 antibody
      • MGC131931 antibody
      • MGC148073 antibody
      • MGC148074 antibody
      • Protein Ric-8A antibody
      • resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) antibody
      • RIC8 antibody
      • RIC8A antibody
      • RIC8A_HUMAN antibody
      • Synembryn-A antibody
      see all

    Images

    • Western blot - Anti-RIC8A antibody (ab97808)
      Western blot - Anti-RIC8A antibody (ab97808)
      All lanes : Anti-RIC8A antibody (ab97808) at 1/1000 dilution

      Lane 1 : A431 whole cell lysate
      Lane 2 : HeLa whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 60 kDa



      7.5% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIC8A antibody (ab97808)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIC8A antibody (ab97808)
      ab97808, at a 1/100 dilution, staining RIC8A in paraffin embedded Adrenal cortical tumor by Immunohistochemistry.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab97808? Please let us know so that we can cite the reference in this datasheet.

    ab97808 has been referenced in 3 publications.

    • Zeng H  et al. Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC. Elife 8:N/A (2019). PubMed: 31741433
    • Chishiki K  et al. Ric-8A, an activator protein of Gai, controls mammalian epithelial cell polarity for tight junction assembly and cystogenesis. Genes Cells 22:293-309 (2017). PubMed: 28185378
    • Boularan C  et al. Resistance to inhibitors of cholinesterase (Ric)-8A and Gai contribute to cytokinesis abscission by controlling vacuolar protein-sorting (Vps)34 activity. PLoS One 9:e86680 (2014). WB, IP, ICC/IF ; Human . PubMed: 24466196

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