Product nameAnti-RIC8A antibody
See all RIC8A primary antibodies
DescriptionRabbit polyclonal to RIC8A
Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to a region within amino acids 528 and 537 of Human RIC8A (NP_068751).
- A431, H1299, HeLa, HepG2 and Raji cell lines; Adrenal cortical tumor
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 24466196|
|IP||Use at an assay dependent concentration. PubMed: 24466196|
|IHC-P||1/100 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 60 kDa.|
FunctionGuanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.
Sequence similaritiesBelongs to the synembryn family.
Cellular localizationCytoplasm. Cell membrane. Colocalizes with RIC8A in CA2 hippocampal neurons. Colocalizes with GNAI1 and RGS14 at the plasma membrane (By similarity).
- Information by UniProt
- MGC104517 antibody
- MGC131931 antibody
- MGC148073 antibody
All lanes : Anti-RIC8A antibody (ab97808) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 60 kDa
7.5% SDS PAGE
ab97808, at a 1/100 dilution, staining RIC8A in paraffin embedded Adrenal cortical tumor by Immunohistochemistry.
This product has been referenced in:
- Chishiki K et al. Ric-8A, an activator protein of Gai, controls mammalian epithelial cell polarity for tight junction assembly and cystogenesis. Genes Cells 22:293-309 (2017). Read more (PubMed: 28185378) »
- Boularan C et al. Resistance to inhibitors of cholinesterase (Ric)-8A and Gai contribute to cytokinesis abscission by controlling vacuolar protein-sorting (Vps)34 activity. PLoS One 9:e86680 (2014). WB, IP, ICC/IF ; Human . Read more (PubMed: 24466196) »