Overview

  • Product name
    Anti-Ricin toxin A chain antibody
  • Description
    Rabbit polyclonal to Ricin toxin A chain
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Electron Microscopy, ELISA, WBmore details
  • Species reactivity
    Reacts with Ricinus communis. Not yet tested in other species.
  • Immunogen

    Full length native protein: Purified intact A chain from RCA 60.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 0.0268% PBS, 2.9% Sodium chloride
  • Purity
    Whole antiserum
  • Purification notes
    sterile-filtered (0.22 mm pore size) serum with the addition of 15 mM NaN3
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy Use at an assay dependent concentration. PubMed: 21886775
ELISA 1/20000.
WB 1/2000. Predicted molecular weight: 32 kDa.

Target

  • Relevance
    Ricin chain A (RTA) is a ribosome inactivating enzyme with a molecular weight of 32 kDa. RTA is normally linked by a disulfide bond to the galactose/N acetylgalactosamine binding lectin (34 kDa), also called the B chain or RTB. Together with RTB, RTA constitutes the heterodimeric cytotoxin Ricin (RCA 60) from Ricinus communis. This ricin, which is a type 2 RIP (ribosome inactivating protein) is among the most potent cytotoxins in nature. Ricin is highly toxic to animal cells and to a lesser extent to plant cells.
  • Alternative names
    • Ricin A chain antibody
    • Ricin antibody

References

This product has been referenced in:
  • Marszalowicz GP  et al. GUCY2C lysosomotropic endocytosis delivers immunotoxin therapy to metastatic colorectal cancer. Oncotarget 5:9460-71 (2014). Read more (PubMed: 25294806) »
  • Wang W  et al. Mannosidase 2, alpha 1 Deficiency Is Associated with Ricin Resistance in Embryonic Stem (ES) Cells. PLoS One 6:e22993 (2011). Electron Microscopy . Read more (PubMed: 21886775) »
See all 4 Publications for this product

Customer reviews and Q&As

Answer

I have found an article on the Internet which indicates very different structures between Ricin Communis Agglutinin and Ricin (toxin). Biol Chem. 1985 Dec 15;260(29):15682-6. The primary sequence of Ricinus communis agglutinin. Comparison with ricin. Roberts LM, Lamb FI, Pappin DJ, Lord JM. A mixture of synthetic oligonucleotides representing all possible sequences of a peptide present in the ricin B chain has been used to screen a cDNA library constructed using ripening castor bean seed poly(A+) RNA. The eight largest recombinant plasmids selected, by hybridization, a single mRNA species whose translational product was identified as preprolectin by immunoprecipitation. Restriction enzyme analysis of these clones demonstrated that two classes were present representing sequences complementary to two distinct but closely related preprolectin mRNA species. The nucleotide sequence of the cloned cDNA from one of these classes encodes preproricin and has been presented elsewhere (Lamb, F. I., Roberts, L. M., and Lord, J. M., (1985) Eur. J. Biochem. 148, 265-270). The nucleotide sequence of the second class is presented here and shown to represent prepro-Ricinus communis agglutinin. The entire coding sequence was deduced from two overlapping cDNA clones having inserts of 1668 and 1151 base pairs. The coding region defines a preproprotein with a 24-amino acid N-terminal signal sequence preceding the A chain (266 amino acids) which is joined to the B chain (262 amino acids) by a 12-amino acid linking peptide. The protein was confirmed as R. communis agglutinin since the deduced B chain N-terminal sequence corresponds exactly with that determined for purified R. communis agglutinin B chain over a region where several residue differences occur in the ricin B chain. The nucleotide and deduced amino acid sequences of the R. communis agglutinin precursor are compared with those of the ricin precursor. I therefore doubt very much that antibodies specific for the toxin (RTA,RTB) will recognise the agglutinin (RCA). I have been informed that ab18246 Rabbit polyclonal to Ricin communis (HRP) and that ab19996 Mouse monoclonal [CP23] to Ricin communis will recognise the RCA protein and this information will be updated on the datasheet very soon. The antibodies ab23515, ab27169, ab27170 are against the toxin chains. I hope this information will help you, please do not hesitate to contact me again if you need further help,

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up