Anti-Ricin toxin B chain antibody (ab27170)

Thank you for your enquiry regarding ab27170. This antibody has been tested by Western blotting on denatured RCA 60 but not in plant samples, therefore we do not know if it will give cross reactivity with plant proteins, sorry I cannot help you more to find out if the antibody will give you similar problems as your previous RCA60 antibody. We currently do not have production plans for a monoclonal antibody against ricin toxin B chain, if you register your area of interrest you can receive an e-mail when the antibody is made and is in our catalogue. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

I have found an article on the Internet which indicates very different structures between Ricin Communis Agglutinin and Ricin (toxin). Biol Chem. 1985 Dec 15;260(29):15682-6. The primary sequence of Ricinus communis agglutinin. Comparison with ricin. Roberts LM, Lamb FI, Pappin DJ, Lord JM. A mixture of synthetic oligonucleotides representing all possible sequences of a peptide present in the ricin B chain has been used to screen a cDNA library constructed using ripening castor bean seed poly(A+) RNA. The eight largest recombinant plasmids selected, by hybridization, a single mRNA species whose translational product was identified as preprolectin by immunoprecipitation. Restriction enzyme analysis of these clones demonstrated that two classes were present representing sequences complementary to two distinct but closely related preprolectin mRNA species. The nucleotide sequence of the cloned cDNA from one of these classes encodes preproricin and has been presented elsewhere (Lamb, F. I., Roberts, L. M., and Lord, J. M., (1985) Eur. J. Biochem. 148, 265-270). The nucleotide sequence of the second class is presented here and shown to represent prepro-Ricinus communis agglutinin. The entire coding sequence was deduced from two overlapping cDNA clones having inserts of 1668 and 1151 base pairs. The coding region defines a preproprotein with a 24-amino acid N-terminal signal sequence preceding the A chain (266 amino acids) which is joined to the B chain (262 amino acids) by a 12-amino acid linking peptide. The protein was confirmed as R. communis agglutinin since the deduced B chain N-terminal sequence corresponds exactly with that determined for purified R. communis agglutinin B chain over a region where several residue differences occur in the ricin B chain. The nucleotide and deduced amino acid sequences of the R. communis agglutinin precursor are compared with those of the ricin precursor. I therefore doubt very much that antibodies specific for the toxin (RTA,RTB) will recognise the agglutinin (RCA). I have been informed that ab18246 Rabbit polyclonal to Ricin communis (HRP) and that ab19996 Mouse monoclonal [CP23] to Ricin communis will recognise the RCA protein and this information will be updated on the datasheet very soon. The antibodies ab23515, ab27169, ab27170 are against the toxin chains. I hope this information will help you, please do not hesitate to contact me again if you need further help,