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    ricin-toxin-b-chain-antibody-ab27170.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation
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Anti-Ricin toxin B chain antibody (ab27170)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (2)

Product price, shipping and contact information

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Abpromise

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Key features and details

  • Rabbit polyclonal to Ricin toxin B chain
  • Suitable for: ELISA, WB
  • Isotype: IgG

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Overview

  • Product name

    Anti-Ricin toxin B chain antibody
  • Description

    Rabbit polyclonal to Ricin toxin B chain
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Full length native protein: Purified intact B chain (RTB) from RCA 60.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 0.0268% PBS, 2.9% Sodium chloride
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Purification notes

    sterile-filtered (0.22 mm pore size) serum with the addition of 15 mM NaN3.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Cell Biology
    • Other Antibodies
    • Plant related targets

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab27170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

Target

  • Alternative names

    • (EC 3.2.2.22) antibody
    • Linker peptide antibody
    • RCA 60 antibody
    • Ricin B chain antibody
    • Ricin chain B antibody
    • Ricin precursor antibody
    • rRNA N-glycosidase antibody
    • RT B antibody
    • RTB antibody
    see all

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab27170? Please let us know so that we can cite the reference in this datasheet.

ab27170 has been referenced in 2 publications.

  • Firsov A  et al. Expression and Immunogenicity of M2e Peptide of Avian Influenza Virus H5N1 Fused to Ricin Toxin B Chain Produced in Duckweed Plants. Front Chem 6:22 (2018). ELISA . PubMed: 29487846
  • Ray S  et al. Modulation of toxin stability by 4-phenylbutyric Acid and negatively charged phospholipids. PLoS One 6:e23692 (2011). PubMed: 21887297

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Do these polyclonal antibodies cross-react with plant proteins? I am trying to express the ricin toxin B chain (RTB) in transgenic potato plants and need to verify the presence of the B chain by Western blot without getting cross-reactivity to other plant proteins in the crude extract. I have polyclonal antibodies against the whole ricin molecule (A and B chain) from Sigma, but they cross react with potato plant proteins--multiple bands appear, even in my untransformed (-) control plants. Are your anti-RTB antibodies raised in animals injected with purified RTB from castor beans or from recombinant RTB? Castor bean plant proteins might generate additional antibodies that could potentially cross-react with other plants, such as potato. Are there any plans to create a monoclonal Ab against RTB that might solve the problem of cross-reactivity?

Read More

Abcam community

Verified customer

Asked on Jan 06 2006

Answer

Thank you for your enquiry regarding ab27170. This antibody has been tested by Western blotting on denatured RCA 60 but not in plant samples, therefore we do not know if it will give cross reactivity with plant proteins, sorry I cannot help you more to find out if the antibody will give you similar problems as your previous RCA60 antibody. We currently do not have production plans for a monoclonal antibody against ricin toxin B chain, if you register your area of interrest you can receive an e-mail when the antibody is made and is in our catalogue. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Jan 06 2006

Question

I have a question about your ricin antibodies. So, if you could answer it or kindly forward it to your tech support, I would truly appreciate it. There are two very similar proteins in castor, ricin (toxin) and RCA (Ricinus communis agglutinin). I would like to know if your antibodies can distinguish the two proteins. Looking forward to hearing from you soon. Thank you.

Read More

Abcam community

Verified customer

Asked on Dec 28 2005

Answer

I have found an article on the Internet which indicates very different structures between Ricin Communis Agglutinin and Ricin (toxin). Biol Chem. 1985 Dec 15;260(29):15682-6. The primary sequence of Ricinus communis agglutinin. Comparison with ricin. Roberts LM, Lamb FI, Pappin DJ, Lord JM. A mixture of synthetic oligonucleotides representing all possible sequences of a peptide present in the ricin B chain has been used to screen a cDNA library constructed using ripening castor bean seed poly(A+) RNA. The eight largest recombinant plasmids selected, by hybridization, a single mRNA species whose translational product was identified as preprolectin by immunoprecipitation. Restriction enzyme analysis of these clones demonstrated that two classes were present representing sequences complementary to two distinct but closely related preprolectin mRNA species. The nucleotide sequence of the cloned cDNA from one of these classes encodes preproricin and has been presented elsewhere (Lamb, F. I., Roberts, L. M., and Lord, J. M., (1985) Eur. J. Biochem. 148, 265-270). The nucleotide sequence of the second class is presented here and shown to represent prepro-Ricinus communis agglutinin. The entire coding sequence was deduced from two overlapping cDNA clones having inserts of 1668 and 1151 base pairs. The coding region defines a preproprotein with a 24-amino acid N-terminal signal sequence preceding the A chain (266 amino acids) which is joined to the B chain (262 amino acids) by a 12-amino acid linking peptide. The protein was confirmed as R. communis agglutinin since the deduced B chain N-terminal sequence corresponds exactly with that determined for purified R. communis agglutinin B chain over a region where several residue differences occur in the ricin B chain. The nucleotide and deduced amino acid sequences of the R. communis agglutinin precursor are compared with those of the ricin precursor. I therefore doubt very much that antibodies specific for the toxin (RTA,RTB) will recognise the agglutinin (RCA). I have been informed that ab18246 Rabbit polyclonal to Ricin communis (HRP) and that ab19996 Mouse monoclonal [CP23] to Ricin communis will recognise the RCA protein and this information will be updated on the datasheet very soon. The antibodies ab23515, ab27169, ab27170 are against the toxin chains. I hope this information will help you, please do not hesitate to contact me again if you need further help,

Read More

Abcam Scientific Support

Answered on Dec 29 2005

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