• Product name
    Anti-RICTOR antibody [7B3]
    See all RICTOR primary antibodies
  • Description
    Mouse monoclonal [7B3] to RICTOR
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human, Monkey
  • Immunogen

    Purified recombinant fragment of Human RICTOR expressed in E. Coli.

  • Positive control
    • HeLa, PANC-1, MOLT4, HepG2 and HEK293 cell lysates. Thyroid gland tissues and Human breast carcinoma. NIH3T3 cells.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR173148-16 are from Tissue Culture Supernatant



Our Abpromise guarantee covers the use of ab104838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 192 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200 - 1/1000.
ELISA 1/10000.


  • Function
    Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
  • Sequence similarities
    Belongs to the RICTOR family.
  • Post-translational
    Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling.
  • Information by UniProt
  • Database links
  • Alternative names
    • AVO3 antibody
    • AVO3 homolog antibody
    • DKFZp686B11164 antibody
    • hAVO3 antibody
    • KIAA1999 antibody
    • Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae) antibody
    • mAVO3 antibody
    • MGC39830 antibody
    • PIA antibody
    • Pianissimo antibody
    • Rapamycin insensitive companion of mTOR antibody
    • Rapamycin-insensitive companion of mTOR antibody
    • Rictor antibody
    • RICTR antibody
    • RICTR_HUMAN antibody
    • RPTOR independent companion of MTOR complex 2 antibody
    • TORC2 specific protein AVO3 antibody
    see all


  • All lanes : Anti-RICTOR antibody [7B3] (ab104838) at 1/500 dilution

    Lane 1 : HeLa cell lysate.
    Lane 2 : PANC-1 cell lysate.
    Lane 3 : MOLT4 cell lysate.
    Lane 4 : HepG2 cell lysate.
    Lane 5 : HEK293 cell lysate.

    Predicted band size: 192 kDa

  • ab104838 at 1/200 dilution staining RICTOR in NIH3T3 cells (green) by immunofluorescence. DRAQ5 fluorescent DNA dye is shown in blue. Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
  • ab104838 at 1/200 dilution staining RICTOR in paraffin-embedded thyroid gland tissues by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab104838 at 1/200 dilution staining RICTOR in paraffin-fixed Human breast carcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab104838 used for Flow cytometric analysis of HeLa cells (green). The negative control is shown in purple.


This product has been referenced in:
  • Frith JE  et al. Mechanically-sensitive miRNAs bias human mesenchymal stem cell fate via mTOR signalling. Nat Commun 9:257 (2018). Read more (PubMed: 29343687) »
  • Hütt-Cabezas M  et al. Activation of mTORC1/mTORC2 signaling in pediatric low-grade glioma and pilocytic astrocytoma reveals mTOR as a therapeutic target. Neuro Oncol 15:1604-14 (2013). IHC-P ; Human . Read more (PubMed: 24203892) »

See all 2 Publications for this product

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