Overview

  • Product name

  • Description

    Rabbit polyclonal to RICTOR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RICTOR aa 1-50. The exact sequence is proprietary. NP_689969.2
    Database link: Q6R327

  • Positive control

    • IP: HEK-293T whole cell lysate. IHC-P: Human prostate carcinoma tissue.
  • General notes

    ab245371 has not performed satisfactorily when used for WB of RICTOR in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RICTOR.

Properties

Applications

Our Abpromise guarantee covers the use of ab245371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
    • Sequence similarities

      Belongs to the RICTOR family.
    • Post-translational
      modifications

      Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling.
    • Information by UniProt
    • Database links

    • Alternative names

      • AVO3 antibody
      • AVO3 homolog antibody
      • DKFZp686B11164 antibody
      • hAVO3 antibody
      • KIAA1999 antibody
      • Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae) antibody
      • mAVO3 antibody
      • MGC39830 antibody
      • PIA antibody
      • Pianissimo antibody
      • Rapamycin insensitive companion of mTOR antibody
      • Rapamycin-insensitive companion of mTOR antibody
      • Rictor antibody
      • RICTR antibody
      • RICTR_HUMAN antibody
      • RPTOR independent companion of MTOR complex 2 antibody
      • TORC2 specific protein AVO3 antibody
      see all

    Images

    • Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for RICTOR using ab245371 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.

    • RICTOR was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245371 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using a different Anti-RICTOR antibody at 1 µg/ml.

      Lane 1: ab245371 IP in HEK-293T whole cell lysate.
      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection: Chemiluminescence with exposure time of 30 seconds.

    References

    ab245371 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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