Overview

  • Product name

    Anti-RICTOR antibody [EPR22008]
    See all RICTOR primary antibodies
  • Description

    Rabbit monoclonal [EPR22008] to RICTOR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RICTOR aa 1650 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6R327

  • Positive control

    • WB: HAP1, HeLa, HEK-293, HCT 116, MDA-MB-231, SW480 and PC-3 whole cell lysate. Flow Cyt: HCT 116 and HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab219950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 200 kDa (predicted molecular weight: 192 kDa).
Flow Cyt 1/500.

Target

  • Function

    Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
  • Sequence similarities

    Belongs to the RICTOR family.
  • Post-translational
    modifications

    Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling.
  • Information by UniProt
  • Database links

  • Alternative names

    • AVO3 antibody
    • AVO3 homolog antibody
    • DKFZp686B11164 antibody
    • hAVO3 antibody
    • KIAA1999 antibody
    • Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae) antibody
    • mAVO3 antibody
    • MGC39830 antibody
    • PIA antibody
    • Pianissimo antibody
    • Rapamycin insensitive companion of mTOR antibody
    • Rapamycin-insensitive companion of mTOR antibody
    • Rictor antibody
    • RICTR antibody
    • RICTR_HUMAN antibody
    • RPTOR independent companion of MTOR complex 2 antibody
    • TORC2 specific protein AVO3 antibody
    see all

Images

  • All lanes : Anti-RICTOR antibody [EPR22008] (ab219950) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate at 20 µg
    Lane 2 : RICTOR knockout HAP1 whole cell lysate at 20 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
    Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 192 kDa



    Blocking/Diluting buffer and concentration: 5%NFDM/TBST.

    Exposure time: 26 seconds.

    ab219950 was shown to specifically react with RICTOR in wild-type HAP1 cells as signal was lost in RICTOR knockout cells. Wild-type and RICTOR knockout samples were subjected to SDS-PAGE. ab219950 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/50,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

  • All lanes : Anti-RICTOR antibody [EPR22008] (ab219950) at 1/1000 dilution

    Lane 1 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
    Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 4 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 2-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 192 kDa



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lane 2: 48 seconds; Lane 3-4: 26 seconds.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling RICTOR with ab219950 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HCT 116 (human colorectal carcinoma cell line) cell line labeling RICTOR with ab219950 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

References

ab219950 has not yet been referenced specifically in any publications.

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