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    rida-antibody-ab151292.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation
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Anti-RIDA antibody (ab151292)

  • Datasheet
  • SDS
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Western blot - Anti-RIDA antibody (ab151292)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (ab151292)

Key features and details

  • Rabbit polyclonal to RIDA
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human RIDA protein (ab101632)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-RIDA antibody
    See all RIDA primary antibodies
  • Description

    Rabbit polyclonal to RIDA
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human RIDA aa 1-137.
    Database link: P52758

  • Positive control

    • A549, HepG2 and HCT116 whole cell lysates; Human DLD1 xenograft tissue.
  • General notes

     This product was previously labelled as HRSP12

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human RIDA protein (ab101632)
  • Related Products

    • Recombinant Human RIDA protein (ab101632)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Rat
Cow
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 14 kDa.
IHC-P
1/500 - 1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 14 kDa.
IHC-P
1/500 - 1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Endoribonuclease responsible for the inhibition of the translation by cleaving mRNA. Inhibits cell-free protein synthesis. Cleaves phosphodiester bonds only in single-stranded RNA.
  • Tissue specificity

    Hepatocytes and renal distal tubular epithelial cells. Only weak expression in other tissues.
  • Sequence similarities

    Belongs to the RutC family.
  • Developmental stage

    Up-regulated during cellular differentiation.
  • Cellular localization

    Cytoplasm. Nucleus. Mostly cytoplasmic but, in less differentiated cells occasionally nuclear.
  • Target information above from: UniProt accession P52758 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 504390 Cow
    • Entrez Gene: 10247 Human
    • Entrez Gene: 65151 Rat
    • Omim: 602487 Human
    • SwissProt: Q3T114 Cow
    • SwissProt: P52758 Human
    • SwissProt: P52759 Rat
    • Unigene: 18426 Human
    • Unigene: 6987 Rat
    see all
  • Alternative names

    • 14.5 kDa translational inhibitor protein antibody
    • 2-iminobutanoate/2-iminopropanoate deaminase antibody
    • Heat responsive protein 12 antibody
    • Heat-responsive protein 12 antibody
    • Hrsp12 antibody
    • p14.5 antibody
    • Perchloric acid soluble protein antibody
    • PSP antibody
    • Reactive intermediate imine deaminase A homolog antibody
    • Ribonuclease UK114 antibody
    • RIDA antibody
    • Translational inhibitor p14.5 antibody
    • Translational inhibitor protein p14.5 antibody
    • UK114 antibody
    • UK114 antigen homolog antibody
    • UK114_HUMAN antibody
    see all

Images

  • Western blot - Anti-RIDA antibody (ab151292)
    Western blot - Anti-RIDA antibody (ab151292)
    Anti-RIDA antibody (ab151292) at 1/2000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 14 kDa



    15% SDS PAGE.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (ab151292)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (ab151292)

    Immunohistochemical analysis of paraffin-embedded Human DLD1 xenograft tissue labeling RIDA with ab151292 at 1/500 dilution.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab151292? Please let us know so that we can cite the reference in this datasheet.

ab151292 has not yet been referenced specifically in any publications.

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