Anti-RIDA antibody (ab151292)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RIDA antibody
See all RIDA primary antibodies -
Description
Rabbit polyclonal to RIDA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow
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Immunogen
Recombinant fragment corresponding to Human RIDA aa 1-137.
Database link: P52758 -
Positive control
- A549, HepG2 and HCT116 whole cell lysates; Human DLD1 xenograft tissue.
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General notes
This product was previously labelled as HRSP12
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab151292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 14 kDa. | |
| IHC-P | 1/500 - 1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Endoribonuclease responsible for the inhibition of the translation by cleaving mRNA. Inhibits cell-free protein synthesis. Cleaves phosphodiester bonds only in single-stranded RNA. -
Tissue specificity
Hepatocytes and renal distal tubular epithelial cells. Only weak expression in other tissues. -
Sequence similarities
Belongs to the RutC family. -
Developmental stage
Up-regulated during cellular differentiation. -
Cellular localization
Cytoplasm. Nucleus. Mostly cytoplasmic but, in less differentiated cells occasionally nuclear. - Information by UniProt
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Database links
- Entrez Gene: 504390 Cow
- Entrez Gene: 10247 Human
- Entrez Gene: 65151 Rat
- Omim: 602487 Human
- SwissProt: Q3T114 Cow
- SwissProt: P52758 Human
- SwissProt: P52759 Rat
- Unigene: 18426 Human
see all -
Alternative names
- 14.5 kDa translational inhibitor protein antibody
- 2-iminobutanoate/2-iminopropanoate deaminase antibody
- Heat responsive protein 12 antibody
see all
Images
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Western blot - Anti-RIDA antibody (ab151292)Anti-RIDA antibody (ab151292) at 1/2000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 14 kDa
15% SDS PAGE. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (ab151292)Immunohistochemical analysis of paraffin-embedded Human DLD1 xenograft tissue labeling RIDA with ab151292 at 1/500 dilution.
References
ab151292 has not yet been referenced specifically in any publications.
