Anti-RIDA antibody (ab151292)
Key features and details
- Rabbit polyclonal to RIDA
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RIDA antibody
See all RIDA primary antibodies -
Description
Rabbit polyclonal to RIDA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human RIDA aa 1-137.
Database link: P52758 -
Positive control
- A549, HepG2 and HCT116 whole cell lysates; Human DLD1 xenograft tissue.
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General notes
This product was previously labelled as HRSP12
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Rat
Cow
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 14 kDa.
|
|
| IHC-P |
1/500 - 1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 14 kDa. |
|
IHC-P
1/500 - 1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Endoribonuclease responsible for the inhibition of the translation by cleaving mRNA. Inhibits cell-free protein synthesis. Cleaves phosphodiester bonds only in single-stranded RNA. -
Tissue specificity
Hepatocytes and renal distal tubular epithelial cells. Only weak expression in other tissues. -
Sequence similarities
Belongs to the RutC family. -
Developmental stage
Up-regulated during cellular differentiation. -
Cellular localization
Cytoplasm. Nucleus. Mostly cytoplasmic but, in less differentiated cells occasionally nuclear. - Information by UniProt
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Database links
- Entrez Gene: 504390 Cow
- Entrez Gene: 10247 Human
- Entrez Gene: 65151 Rat
- Omim: 602487 Human
- SwissProt: Q3T114 Cow
- SwissProt: P52758 Human
- SwissProt: P52759 Rat
- Unigene: 18426 Human
see all -
Alternative names
- 14.5 kDa translational inhibitor protein antibody
- 2-iminobutanoate/2-iminopropanoate deaminase antibody
- Heat responsive protein 12 antibody
see all
Images
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Western blot - Anti-RIDA antibody (ab151292)Anti-RIDA antibody (ab151292) at 1/2000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 14 kDa
15% SDS PAGE. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (ab151292)Immunohistochemical analysis of paraffin-embedded Human DLD1 xenograft tissue labeling RIDA with ab151292 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151292 has not yet been referenced specifically in any publications.