Product nameAnti-Rif1 antibody
See all Rif1 primary antibodies
DescriptionRabbit polyclonal to Rif1
SpecificityThe immunogen sequence for ab13422 represents the C-terminal end of mouse Rif1, mouse teleomere-associated protein RIF1 and mouse Rap1-interacting factor.
Tested applicationsSuitable for: IP, ELISA, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Cow, Dog, Human
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab13422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution (PMID 19000314).
WB: 1/500 - 1/3000. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRequired for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints.
Tissue specificityHighly expressed in testis.
Developmental stageExpression peaks in late G2/S phase of the cell cycle.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Chromosome > telomere. Cytoplasm > cytoskeleton > spindle. Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase.
- Information by UniProt
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This product has been referenced in:
- Dan J et al. Rif1 maintains telomere length homeostasis of ESCs by mediating heterochromatin silencing. Dev Cell 29:7-19 (2014). Read more (PubMed: 24735877) »
- Daxinger L et al. An ENU mutagenesis screen identifies novel and known genes involved in epigenetic processes in the mouse. Genome Biol 14:R96 (2013). Read more (PubMed: 24025402) »