Overview

  • Product name

  • Description

    Goat polyclonal to Rif1
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Monkey
  • Immunogen

    Synthetic peptide:

    C-EEGIIDANKTETNT

    (with a Cysteine residue linker), corresponding to internal sequence amino acids 2076-2089 of Human Rif1 (NP_060621.3).

  • Positive control

    • IHC-P: Human Testis and Brain, Cortex tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints.
  • Tissue specificity

    Highly expressed in testis.
  • Developmental stage

    Expression peaks in late G2/S phase of the cell cycle.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Chromosome > telomere. Cytoplasm > cytoskeleton > spindle. Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp781N1478 antibody
    • FLJ10599 antibody
    • FLJ11269 antibody
    • FLJ12870 antibody
    • Rap1 interacting factor 1 antibody
    • Rap1 interacting factor 1 homolog antibody
    • RAP1 interacting factor homolog antibody
    • RAP1 interacting factor homolog (yeast) antibody
    • Rap1-interacting factor 1 homolog antibody
    • Receptor interacting factor 1 antibody
    • RIF 1 antibody
    • RIF1 antibody
    • RIF1 isoform 4 antibody
    • RIF1_HUMAN antibody
    • Telomere associated protein RIF 1 antibody
    • Telomere associated protein RIF1 antibody
    • Telomere-associated protein RIF1 antibody
    • Telomeric protein RIF 1 antibody
    • Telomeric protein RIF1 antibody
    see all

Images

  • Immunohistochemistry analysis of Formalin fixed and Paraffin Embedded (IHC-P) Human Testis tissue labelling Rif1 with ab134812 at 3.75 µg/ml.
  • Immunohistochemistry analysis of Formalin fixed and Paraffin Embedded (IHC-P) Human Brain, Cortex tissue labelling Rif1 with ab134812 at 3.75 µg/ml.

References

ab134812 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab134812.
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