Overview

  • Product name

  • Description

    Goat polyclonal to Rif1
  • Host species

    Goat
  • Specificity

    ab140464 is expected to recognize both reported isoforms (NP_060621.3; NP_001171134.1). The immunizing peptide sequence has been optimised for cross-reactiviy in mouse. Reported variants represent identical protein: NP_001171135.1, NP_001171136.1, NP_001171134.1
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Dog, Pig, Chimpanzee, Macaque monkey
  • Immunogen

    Synthetic peptide

    RNLQSRWRSPSHEN

    (with a Cysteine residue linker) from the C terminal amino acids 2458-2470 of Human Rif1 (NP_060621.3; NP_001171134.1).

  • Positive control

    • HeLa and Mouse D3 (ES) lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab140464 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 270 kDa (predicted molecular weight: 274 kDa).

Target

  • Function

    Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints.
  • Tissue specificity

    Highly expressed in testis.
  • Developmental stage

    Expression peaks in late G2/S phase of the cell cycle.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Chromosome > telomere. Cytoplasm > cytoskeleton > spindle. Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp781N1478 antibody
    • FLJ10599 antibody
    • FLJ11269 antibody
    • FLJ12870 antibody
    • Rap1 interacting factor 1 antibody
    • Rap1 interacting factor 1 homolog antibody
    • RAP1 interacting factor homolog antibody
    • RAP1 interacting factor homolog (yeast) antibody
    • Rap1-interacting factor 1 homolog antibody
    • Receptor interacting factor 1 antibody
    • RIF 1 antibody
    • RIF1 antibody
    • RIF1 isoform 4 antibody
    • RIF1_HUMAN antibody
    • Telomere associated protein RIF 1 antibody
    • Telomere associated protein RIF1 antibody
    • Telomere-associated protein RIF1 antibody
    • Telomeric protein RIF 1 antibody
    • Telomeric protein RIF1 antibody
    see all

Images

  • All lanes : Anti-Rif1 antibody (ab140464) at 1 µg/ml

    Lane 1 : HeLa cell lysate in RIPA buffer
    Lane 2 : HeLa cell lysate in RIPA buffer with Rif1 siRNA
    Lane 3 : Mouse D3 (ES) cell lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 274 kDa



    The lower panel shows the detection of Tubulin in each sample.

References

This product has been referenced in:

  • Alver RC  et al. Reversal of DDK-Mediated MCM Phosphorylation by Rif1-PP1 Regulates Replication Initiation and Replisome Stability Independently of ATR/Chk1. Cell Rep 18:2508-2520 (2017). Read more (PubMed: 28273463) »
See 1 Publication for this product

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