Anti-Rif1 antibody (ab70254)
Key features and details
- Rabbit polyclonal to Rif1
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rif1 antibody
See all Rif1 primary antibodies -
Description
Rabbit polyclonal to Rif1 -
Host species
Rabbit -
Specificity
Reactivity with mouse Rif1 is not expected owing to divergence of sequence between this ortholog and human Rif1 at the site to which the epitope maps. -
Tested applications
Suitable for: IHC-P, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
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Immunogen
Synthetic peptide corresponding to a region between residues 1 and 50 of human Rif1 (NP_060621.3)
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Positive control
- Whole cell lysate from 293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at 1-5 µg/mg of lysate.
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| Notes |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at 1-5 µg/mg of lysate. |
Target
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Function
Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints. -
Tissue specificity
Highly expressed in testis. -
Developmental stage
Expression peaks in late G2/S phase of the cell cycle. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Chromosome > telomere. Cytoplasm > cytoskeleton > spindle. Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase. - Information by UniProt
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Database links
- Entrez Gene: 459657 Chimpanzee
- Entrez Gene: 55183 Human
- Omim: 608952 Human
- SwissProt: Q5UIP0 Human
- Unigene: 655671 Human
- Unigene: 725538 Human
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Alternative names
- DKFZp781N1478 antibody
- FLJ10599 antibody
- FLJ11269 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody (ab70254)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryngeal squamous cell carcinoma tissue labelling Rif1 with ab70254 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-Rif1 antibody (ab70254)Immunoprecipitation analysis of 293T whole cell lysate (1mg for IP, 25% of reaction loaded), using ab70254 at 5 µg/mg lysate for IP. Immunoprecipitated Rif1 was blotted using ab70307 at 0.4µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab70254 has been referenced in 2 publications.
- Hiraga SI et al. Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation. EMBO Rep 18:403-419 (2017). IP ; Human . PubMed: 28077461
- Alver RC et al. Reversal of DDK-Mediated MCM Phosphorylation by Rif1-PP1 Regulates Replication Initiation and Replisome Stability Independently of ATR/Chk1. Cell Rep 18:2508-2520 (2017). PubMed: 28273463