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Inquiry: Dear sir/madam, Could you please provide more detailed conditions (e.g., lysis buffer used, and wash condition) for the IP experiments from 293T to obtain the data shown in the product page?
Asked on Aug 31 2016
Thank you for getting in touch.
Please find below the details about the IP experiments for ab70254:
IP lysis Buffer (also used as wash buffer)
12.5 ml 1M NaCl (250mM)
2.5 ml 1M Tris (50mM)
500ul 0.5M EDTA (5mM)
2.5ml 10% NP-40
32 ml dH20
500 µl of cell lysate at 1-3 mg/ml are used per IP (performed with protein A).
The washing steps are performed in cold cell lysis buffer three times.
I am happy to answer any further questions you have.
Answered on Aug 31 2016