Product nameAnti-Rif1 antibody - C-terminal
See all Rif1 primary antibodies
DescriptionRabbit polyclonal to Rif1 - C-terminal
Tested applicationsSuitable for: WB, IP, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Rif1 (C terminal). The exact sequence is proprietary.
Database link: Q5UIP0
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HeLa whole cell extract. IHC-P: Human breast carcinoma and cervical carcinoma tissues. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 274 kDa.|
|IP||1/100 - 1/500.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
FunctionRequired for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints.
Tissue specificityHighly expressed in testis.
Developmental stageExpression peaks in late G2/S phase of the cell cycle.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Chromosome > telomere. Cytoplasm > cytoskeleton > spindle. Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase.
- Information by UniProt
- DKFZp781N1478 antibody
- FLJ10599 antibody
- FLJ11269 antibody
All lanes : Anti-Rif1 antibody - C-terminal (ab229656) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 274 kDa
5% SDS-PAGE gel.
4% paraformaldehyde fixed heLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Rif1 (green) using ab229656 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Rif1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 μg ab229656. Western blot was performed from the immunoprecipitate using ab229656. Anti-rabbit IgG was used as secondary antibody.
Lane 1: Control IgG IP instead of ab229656 in HeLa whole cell extract.
Lane 2: ab229656 IP in HeLa whole cell extract.
Paraffin-embedded human breast carcinoma tissue stained for Rif1 using ab229656 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human cervical carcinoma tissue stained for Rif1 using ab229656 at 1/500 dilution in immunohistochemical analysis.
ab229656 has not yet been referenced specifically in any publications.