Overview

  • Product name

    Anti-Rif1 antibody - C-terminal
    See all Rif1 primary antibodies
  • Description

    Rabbit polyclonal to Rif1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Rif1 (C terminal). The exact sequence is proprietary.
    Database link: Q5UIP0

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HeLa whole cell extract. IHC-P: Human breast carcinoma and cervical carcinoma tissues. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 274 kDa.
IP 1/100 - 1/500.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints.
  • Tissue specificity

    Highly expressed in testis.
  • Developmental stage

    Expression peaks in late G2/S phase of the cell cycle.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Chromosome > telomere. Cytoplasm > cytoskeleton > spindle. Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp781N1478 antibody
    • FLJ10599 antibody
    • FLJ11269 antibody
    • FLJ12870 antibody
    • Rap1 interacting factor 1 antibody
    • Rap1 interacting factor 1 homolog antibody
    • RAP1 interacting factor homolog antibody
    • RAP1 interacting factor homolog (yeast) antibody
    • Rap1-interacting factor 1 homolog antibody
    • Receptor interacting factor 1 antibody
    • RIF 1 antibody
    • RIF1 antibody
    • RIF1 isoform 4 antibody
    • RIF1_HUMAN antibody
    • Telomere associated protein RIF 1 antibody
    • Telomere associated protein RIF1 antibody
    • Telomere-associated protein RIF1 antibody
    • Telomeric protein RIF 1 antibody
    • Telomeric protein RIF1 antibody
    see all

Images

  • All lanes : Anti-Rif1 antibody - C-terminal (ab229656) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 274 kDa



    5% SDS-PAGE gel.

  • 4% paraformaldehyde fixed heLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Rif1 (green) using ab229656 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • Rif1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 μg ab229656. Western blot was performed from the immunoprecipitate using ab229656. Anti-rabbit IgG was used as secondary antibody.

    Lane 1: Control IgG IP instead of ab229656 in HeLa whole cell extract.

    Lane 2: ab229656 IP in HeLa whole cell extract.

  • Paraffin-embedded human breast carcinoma tissue stained for Rif1 using ab229656 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded human cervical carcinoma tissue stained for Rif1 using ab229656 at 1/500 dilution in immunohistochemical analysis.

References

ab229656 has not yet been referenced specifically in any publications.

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