Key features and details
- Rabbit polyclonal to Rif1 - C-terminal
- Suitable for: WB, IP, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Rif1 antibody - C-terminal
See all Rif1 primary antibodies
DescriptionRabbit polyclonal to Rif1 - C-terminal
Tested applicationsSuitable for: WB, IP, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Rif1 (C terminal). The exact sequence is proprietary.
Database link: Q5UIP0
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HeLa whole cell extract. IHC-P: Human breast carcinoma and cervical carcinoma tissues. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 274 kDa.|
|IP||1/100 - 1/500.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
FunctionRequired for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints.
Tissue specificityHighly expressed in testis.
Developmental stageExpression peaks in late G2/S phase of the cell cycle.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Chromosome > telomere. Cytoplasm > cytoskeleton > spindle. Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase.
- Information by UniProt
- DKFZp781N1478 antibody
- FLJ10599 antibody
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All lanes : Anti-Rif1 antibody - C-terminal (ab229656) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 274 kDa
5% SDS-PAGE gel.
4% paraformaldehyde fixed heLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Rif1 (green) using ab229656 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Rif1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 μg ab229656. Western blot was performed from the immunoprecipitate using ab229656. Anti-rabbit IgG was used as secondary antibody.
Lane 1: Control IgG IP instead of ab229656 in HeLa whole cell extract.
Lane 2: ab229656 IP in HeLa whole cell extract.
Paraffin-embedded human breast carcinoma tissue stained for Rif1 using ab229656 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human cervical carcinoma tissue stained for Rif1 using ab229656 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229656 has not yet been referenced specifically in any publications.