Product nameAnti-RIG-I/DDX58 antibody
See all RIG-I/DDX58 primary antibodies
DescriptionRabbit polyclonal to RIG-I/DDX58
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Full length protein corresponding to Human RIG-I/DDX58.
- C2C12 cell lysate. Human uterus and mouse heart tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab45428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 107 kDa).|
FunctionInvolved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.
Tissue specificityPresent in vascular smooth cells (at protein level).
Sequence similaritiesBelongs to the helicase family.
Contains 2 CARD domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
DomainThe repressor domain controls homomultimerization and interaction with MAVS.
The helicase domain is responsible for dsRNA recognition.
The 2 CARD domains are responsible for interaction with and signaling through MAVS.
The second CARD domain is the primary site for 'Lys-63'-linked ubiquitination.
modificationsIsgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation.
Ubiquitinated. Undergoes 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS binding and to induce anti-viral signal transduction. Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated ubiquitination. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains.
Cellular localizationCytoplasm. Colocalized with TRIM25 at cytoplasmic perinuclear bodies.
- Information by UniProt
- Ddx58 antibody
- DDX58_HUMAN antibody
- DEAD (Asp Glu Ala Asp) box polypeptide 58 antibody
Lane 1 : Anti-RIG-I/DDX58 antibody (ab45428) at 0.5 µg/ml
Lane 2 : Anti-RIG-I/DDX58 antibody (ab45428) at 1 µg/ml
Lane 3 : Anti-RIG-I/DDX58 antibody (ab45428) at 2 µg/ml
All lanes : C2C12 cell lysate
Predicted band size: 107 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab45428 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45428, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab45428 at 5 µg/ml staining RIG-I/DDX58 in human heart tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
This product has been referenced in:
- Lee W et al. Vibrio vulnificus quorum-sensing molecule cyclo(Phe-Pro) inhibits RIG-I-mediated antiviral innate immunity. Nat Commun 9:1606 (2018). Read more (PubMed: 29686409) »
- Zhu H et al. RIG-I antiviral signaling drives interleukin-23 production and psoriasis-like skin disease. EMBO Mol Med 9:589-604 (2017). WB, IHC-P, Flow Cyt ; Mouse . Read more (PubMed: 28377495) »