Overview

  • Product name

  • Description

    Rabbit polyclonal to RIL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow
  • Immunogen

    Synthetic peptide corresponding to Human RIL aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab95864)

  • Positive control

    • This antibody gave a positive signal in A431 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab90157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).

Target

Images

  • Anti-RIL antibody (ab90157) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    RIL contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References

ab90157 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab90157.
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