Product nameAnti-RIL antibody
See all RIL primary antibodies
DescriptionRabbit polyclonal to RIL
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow
Synthetic peptide corresponding to Human RIL aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in A431 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab90157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).|
Tissue specificityIsoform 2 is found in brain.
Sequence similaritiesContains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.
modificationsPhosphorylated on tyrosine residue(s). Can be dephosphorylated by PTPN13.
- Information by UniProt
- Enigma homolog antibody
- LIM domain protein antibody
- LIM domain protein RIL antibody
Anti-RIL antibody (ab90157) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
RIL contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ab90157 has not yet been referenced specifically in any publications.