Key features and details
- Rabbit polyclonal to RIL
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RIL antibody
See all RIL primary antibodies
DescriptionRabbit polyclonal to RIL
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow
Synthetic peptide corresponding to Human RIL aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in A431 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab90157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).|
Tissue specificityIsoform 2 is found in brain.
Sequence similaritiesContains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.
modificationsPhosphorylated on tyrosine residue(s). Can be dephosphorylated by PTPN13.
- Information by UniProt
- Enigma homolog antibody
- LIM domain protein antibody
- LIM domain protein RIL antibody
Anti-RIL antibody (ab90157) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
RIL contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ab90157 has not yet been referenced specifically in any publications.