Recombinant Anti-RIL antibody [EPR16199] (ab197026)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16199] to RIL
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-RIL antibody [EPR16199]
See all RIL primary antibodies -
Description
Rabbit monoclonal [EPR16199] to RIL -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human RIL aa 50-250. The exact sequence is proprietary.
Database link: P50479 -
Positive control
- PC-3, A431 and SH-SY5Y whole cell lysates; SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16199 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab197026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). |
Target
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Tissue specificity
Isoform 2 is found in brain. -
Sequence similarities
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
Post-translational
modificationsPhosphorylated on tyrosine residue(s). Can be dephosphorylated by PTPN13. - Information by UniProt
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Database links
- Entrez Gene: 8572 Human
- Omim: 603422 Human
- SwissProt: P50479 Human
- Unigene: 424312 Human
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Alternative names
- Enigma homolog antibody
- LIM domain protein antibody
- LIM domain protein RIL antibody
see all
Images
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Anti-RIL antibody [EPR16199] (ab197026) at 1/1000 dilution + PC-3 (Human prostate cancer cell line) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-RIL antibody [EPR16199] (ab197026) at 1/1000 dilution + A431 (Human epidermoid carcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-RIL antibody [EPR16199] (ab197026) at 1/10000 dilution + SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Datasheets and documents
Certificate of Compliance
References (0)
ab197026 has not yet been referenced specifically in any publications.