Recombinant Anti-RIL antibody [EPR16199] (ab197026)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RIL antibody [EPR16199]
See all RIL primary antibodies -
Description
Rabbit monoclonal [EPR16199] to RIL -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human RIL aa 50-250. The exact sequence is proprietary.
Database link: P50479 -
Positive control
- PC-3, A431 and SH-SY5Y whole cell lysates; SH-SY5Y cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16199 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab197026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). | |
| Flow Cyt | 1/140. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Tissue specificity
Isoform 2 is found in brain. -
Sequence similarities
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
Post-translational
modificationsPhosphorylated on tyrosine residue(s). Can be dephosphorylated by PTPN13. - Information by UniProt
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Database links
- Entrez Gene: 8572 Human
- Omim: 603422 Human
- SwissProt: P50479 Human
- Unigene: 424312 Human
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Alternative names
- Enigma homolog antibody
- LIM domain protein antibody
- LIM domain protein RIL antibody
see all
Images
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![Western blot - Anti-RIL antibody [EPR16199] (ab197026)](https://www.abcam.com/ps/products/197/ab197026/Images/ab197026-236855-anti-ril-antibody-epr16199-western-blot.jpg)
Western blot - Anti-RIL antibody [EPR16199] (ab197026)Anti-RIL antibody [EPR16199] (ab197026) at 1/1000 dilution + PC-3 (Human prostate cancer cell line) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-RIL antibody [EPR16199] (ab197026)](https://www.abcam.com/ps/products/197/ab197026/Images/ab197026-236856-anti-ril-antibody-epr16199-western-blot.jpg)
Western blot - Anti-RIL antibody [EPR16199] (ab197026)Anti-RIL antibody [EPR16199] (ab197026) at 1/1000 dilution + A431 (Human epidermoid carcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-RIL antibody [EPR16199] (ab197026)](https://www.abcam.com/ps/products/197/ab197026/Images/ab197026-236857-ab197026WBc.jpg)
Western blot - Anti-RIL antibody [EPR16199] (ab197026)Anti-RIL antibody [EPR16199] (ab197026) at 1/10000 dilution + SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Datasheets and documents
References
ab197026 has not yet been referenced specifically in any publications.
