Anti-RILP antibody (ab140188)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RILP antibody
See all RILP primary antibodies -
Description
Rabbit polyclonal to RILP -
Host species
Rabbit -
Specificity
At least two isoforms of RILP are known to exist; ab140188 will only detect the longer isoform. -
Tested applications
Suitable for: ICC/IF, WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
An 18 amino acid synthetic peptide near the center of Human RILP.
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Positive control
- A20 cell lysate; HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab140188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. | |
| ICC | Use a concentration of 2.5 µg/ml. |
Target
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Function
Rab effector playing a role in late endocytic transport to degradative compartments. Involved in the regulation of lysosomal morphology and distribution. Induces recruitment of dynein-dynactin motor complexes to Rab7A-containing late endosome and lysosome compartments. Promotes centripetal migration of phagosomes and the fusion of phagosomes with the late endosomes and lysosomes. -
Tissue specificity
Ubiquitous. Strongly expressed in fetal heart, heart, stomach, spleen, adrenal gland, thyroid gland, salivary gland, fetal liver, liver and lung. Poorly expressed in brain. -
Sequence similarities
Contains 1 RILP-like domain. -
Cellular localization
Endomembrane system. Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle > phagosome membrane. Associated with late endosomal, lysosomal and phagosomal membranes. The interaction with RAB7A is necessary for its recruitment to phagosomes. - Information by UniProt
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Database links
- Entrez Gene: 83547 Human
- Entrez Gene: 280408 Mouse
- Omim: 607848 Human
- SwissProt: Q96NA2 Human
- SwissProt: Q5ND29 Mouse
- Unigene: 534497 Human
- Unigene: 41416 Mouse
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Alternative names
- FLJ31193 antibody
- PP10141 antibody
- Rab interacting lysosomal protein antibody
see all
Images
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Western blot - Anti-RILP antibody (ab140188)All lanes : Anti-RILP antibody (ab140188) at 1 µg/ml
Lane 1 : A20 cell lysate
Lane 2 : A20 cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 44 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-RILP antibody (ab140188)Immunofluorescence of RILP in HeLa cells with ab140188 at 20 ug/mL. -
Immunocytochemistry - Anti-RILP antibody (ab140188)Immunocytochemical analysis of HeLa cells labelling RILP with ab140188 at 2.5 µg/ml.
Datasheets and documents
References
ab140188 has not yet been referenced specifically in any publications.
