Anti-RILP antibody (ab140188)

Submit a review Submit a question References (1)
Western blot - Anti-RILP antibody (ab140188)
  • Immunocytochemistry/ Immunofluorescence - Anti-RILP antibody (ab140188)
  • Immunocytochemistry - Anti-RILP antibody (ab140188)

Key features and details

  • Rabbit polyclonal to RILP
  • Suitable for: ICC/IF, WB, ICC
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-RILP antibody

  • Description

    Rabbit polyclonal to RILP

  • Host species

    Rabbit

  • Specificity

    At least two isoforms of RILP are known to exist; ab140188 will only detect the longer isoform.

  • Tested applications

    Suitable for: ICC/IF, WB, ICCmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    An 18 amino acid synthetic peptide near the center of Human RILP.

  • Positive control

    • A20 cell lysate; HeLa cells

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 20 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
ICC
Use a concentration of 2.5 µg/ml.
Notes
ICC/IF
Use a concentration of 20 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
ICC
Use a concentration of 2.5 µg/ml.

Target

  • Function

    Rab effector playing a role in late endocytic transport to degradative compartments. Involved in the regulation of lysosomal morphology and distribution. Induces recruitment of dynein-dynactin motor complexes to Rab7A-containing late endosome and lysosome compartments. Promotes centripetal migration of phagosomes and the fusion of phagosomes with the late endosomes and lysosomes.

  • Tissue specificity

    Ubiquitous. Strongly expressed in fetal heart, heart, stomach, spleen, adrenal gland, thyroid gland, salivary gland, fetal liver, liver and lung. Poorly expressed in brain.

  • Sequence similarities

    Contains 1 RILP-like domain.

  • Cellular localization

    Endomembrane system. Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle > phagosome membrane. Associated with late endosomal, lysosomal and phagosomal membranes. The interaction with RAB7A is necessary for its recruitment to phagosomes.
  • Information by UniProt

  • Database links

    • Alternative names

      • FLJ31193 antibody
      • PP10141 antibody
      • Rab interacting lysosomal protein antibody
      • Rab-interacting lysosomal protein antibody
      • Rilp antibody
      • RILP_HUMAN antibody
      see all

    Images

    • Western blot - Anti-RILP antibody (ab140188)
      Western blot - Anti-RILP antibody (ab140188)
      All lanes : Anti-RILP antibody (ab140188) at 1 µg/ml

      Lane 1 : A20 cell lysate
      Lane 2 : A20 cell lysate with blocking peptide

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 44 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-RILP antibody (ab140188)
      Immunocytochemistry/ Immunofluorescence - Anti-RILP antibody (ab140188)
      Immunofluorescence of RILP in HeLa cells with ab140188 at 20 ug/mL.
    • Immunocytochemistry - Anti-RILP antibody (ab140188)
      Immunocytochemistry - Anti-RILP antibody (ab140188)
      Immunocytochemical analysis of HeLa cells labelling RILP with ab140188 at 2.5 µg/ml.

    References (1)

    Publishing research using ab140188? Please let us know so that we can cite the reference in this datasheet.

    ab140188 has been referenced in 1 publication.

    • Zhao Y  et al. Dihydroartemisinin Ameliorates Learning and Memory in Alzheimer's Disease Through Promoting Autophagosome-Lysosome Fusion and Autolysosomal Degradation for Aß Clearance. Front Aging Neurosci 12:47 (2020). PubMed: 32210783

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab140188.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com