Overview

  • Product name
  • Description
    Rabbit polyclonal to RILP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to C terminal residues of human RILP.

  • Positive control
    • transient ever-expressed cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab54559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa). for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Rab effector playing a role in late endocytic transport to degradative compartments. Involved in the regulation of lysosomal morphology and distribution. Induces recruitment of dynein-dynactin motor complexes to Rab7A-containing late endosome and lysosome compartments. Promotes centripetal migration of phagosomes and the fusion of phagosomes with the late endosomes and lysosomes.
  • Tissue specificity
    Ubiquitous. Strongly expressed in fetal heart, heart, stomach, spleen, adrenal gland, thyroid gland, salivary gland, fetal liver, liver and lung. Poorly expressed in brain.
  • Sequence similarities
    Contains 1 RILP-like domain.
  • Cellular localization
    Endomembrane system. Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle > phagosome membrane. Associated with late endosomal, lysosomal and phagosomal membranes. The interaction with RAB7A is necessary for its recruitment to phagosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ31193 antibody
    • PP10141 antibody
    • Rab interacting lysosomal protein antibody
    • Rab-interacting lysosomal protein antibody
    • Rilp antibody
    • RILP_HUMAN antibody
    see all

Images

  • Anti-RILP antibody (ab54559) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa
    Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Valapala M  et al. Lysosomal-mediated waste clearance in retinal pigment epithelial cells is regulated by CRYBA1/ßA3/A1-crystallin via V-ATPase-MTORC1 signaling. Autophagy 10:480-96 (2014). Read more (PubMed: 24468901) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

DISCOUNT CODE: **********
Expiration date: DD MM YYYY

I am very pleased to hear you would like to accept our offer and test ab93825in BSC1/CHO cells and IF. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for BSC1/CHO cells and IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us.

I understand your hesitation, but please be reassured that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (www.abcam.com/abpromise).

Although we have tested this antibody mainly with recombinant protein, we are confident that it works for human endogenous protein as well, as you can see from the Western blot with HeLa cells indicated on the datasheet.

Unfortunately, because we carry over 80,000 products, it isn't feasible for us to keep small sample sizes of our products. Having said this however, I am happy to let you know that with your intended experiments you qualify for our testing discount programme:

To our knowledge, ab54559 has not been tested in BSC1 or CHO cells or in IF.

The immunogen (xxxxxxxxxxxxxxx) though exhibits a sequence similarity with the chinese hamster and monkey orthologues of 63% and 90%, respectively. Thus, a cross-reactivity is (more or less) likely.

Therefore, I can offer a discount off a future purchase if you buy ab54559 now, test it in the mentioned cell lines and/or IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab54559. I will then send a discount code. This code must be issued before purchasing ab54559 so please wait for my reply before ordering.

2. Purchase ab54559 either by phone, fax, or online (www.abcam.com).

3. Test it in BSC1/CHO cells/IF.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews .

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab54559 turns out to be unsuitable for the tested conditions, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

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Answer

Thank you very much for your interest in ab54559.

To our knowledge,ab54559 has not been tested in immunoflourescence. Therefore, I can offer a discount off a future purchase if you buyab54559 now, test it inimmunoflourescence and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and testab54559 in immunoflourescence. I will then send a discount code. This code must be issued before purchasing ab54559 so please wait for my reply before ordering.

2. Purchaseab54559 either by phone, fax, or online (www.abcam.com).

3. Test it in immunoflourescence.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifab54559 turns out to be unsuitable for immunoflourescence, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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