Overview

  • Product name

    Anti-RIMBP2 antibody [12.3_1E4]
  • Description

    Mouse monoclonal [12.3_1E4] to RIMBP2
  • Host species

    Mouse
  • Specificity

    ab128045 specificity has been determined by protein microarray.
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Neuroblastoma (SHSY5Y)

  • Positive control

    • Transfected HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    12.3_1E4
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 116 kDa.
IP Use a concentration of 4 µg/ml.

Target

  • Function

    Plays a role in the synaptic transmission as bifunctional linker that interacts simultaneously with RIMS1, RIMS2, CACNA1D and CACNA1B.
  • Sequence similarities

    Belongs to the RIMBP family.
    Contains 3 fibronectin type-III domains.
    Contains 3 SH3 domains.
  • Domain

    The SH3 domains mediate binding to a proline-rich motif in RIMS1, RIMS2, CACNA1D and CACNA1B.
  • Cellular localization

    Cell membrane. Cell junction > synapse. Synaptic plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • RBP2 antibody
    • RIM binding protein 2 antibody
    • RIM-BP2 antibody
    • RIMB2_HUMAN antibody
    • RIMBP2 antibody
    • RIMS-binding protein 2 antibody
    • RP11-662M24.2 antibody
    see all

Images

  • Lanes 1-3 : Anti-RIMBP2 antibody [12.3_1E4] (ab128045) at 1/1000 dilution
    Lanes 4-6 : V5 antibody control at 1/1000 dilution

    Lanes 1 & 4 : Transfected HeLa cell lysate
    Lanes 2 & 5 : Non-transfected HeLa cell lysate
    Lanes 3 & 6 : SH-SY5Y cell lysate

    Predicted band size: 116 kDa

  • 2 µg of ab128045 was added to aliquots of cleared HeLa cell lysates from transfected Human cell lines and incubated for 2 hr at 4°C. Protein G Dynabeads were then added, and lysates were incubated for an additional 2 hr at 4°C to capture the IgG-protein complex. Anti-V5 antibody was used in parallel as a positive control. As a negative control, the primary antibody was excluded.
    Lane 1: Cell Lysate control
    Lane 2: Cell lysate with ab128045
    Lane 3: Cell lysate without ab128045
    Lane 4: V5 tag control

References

ab128045 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab128045.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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