Key features and details
- Rabbit polyclonal to RIN1 - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RIN1 antibody - C-terminal
See all RIN1 primary antibodies
DescriptionRabbit polyclonal to RIN1 - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human RIN1 (C terminal). The exact sequence is proprietary.
Database link: Q13671
- WB: HEK-293T, HeLa and HepG2 whole cell extracts.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 84 kDa.|
RelevanceRIN1 is a Ras effector protein which can affect Ras signaling in a variety of ways. It enhances signaling from ABL1 and ABL2 whcih are involved in regulation of cytoskeletal remodeling. It may function as a guanine nucleotide exchange factor in activation of Rab5a. Rin1 also competes with RAF1 for binding of the activated form of Ras. It may play a role inhibiting the modulation of neuronal plasticity in formation of aversive memory, and has been implicated as a tumor supressor gene in breast cancer.
Cellular localizationCytoplasmic and Plasma membrane
- Ras and Rab interactor 1 antibody
- ras inhibitor 1 antibody
- ras inhibitor antibody
All lanes : Anti-RIN1 antibody - C-terminal (ab229684) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 84 kDa
7.5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229684 has not yet been referenced specifically in any publications.