Overview

  • Product name
    Anti-RIN3 antibody
  • Description
    Rabbit polyclonal to RIN3
  • Host species
    Rabbit
  • Specificity
    ab64838 detects endogenous levels of total RIN3 protein.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human RIN3.

  • Positive control
    • HUVEC and K562 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab64838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).

Target

  • Relevance
    RIN3 belongs to the RIN family of Ras interaction-interference proteins, which are binding partners to the RAB5 small GTPases.
  • Cellular localization
    Cytoplasm. Cytoplasmic vesicle. Early endosome. Note: Activation of tyrosine phosphorylation signaling induces translocation to cytoplasmic vesicles.
  • Database links
    • Alternative names
      • DKFZp762H1613 antibody
      • FLJ11700 antibody
      • FLJ22439 antibody
      • Ras and Rab interactor 3 antibody
      • Ras interaction/interference protein 3 antibody
      see all

    Images

    • Western blot - Anti-RIN3 antibody (ab64838)
      Western blot - Anti-RIN3 antibody (ab64838)
      All lanes : Anti-RIN3 antibody (ab64838) at 1/500 dilution

      Lane 1 : HUVEC cell extract
      Lane 2 : K562 cell extract
      Lane 3 : HUVEC cell extract with immunising peptide

      Predicted band size: 108 kDa
      Observed band size: 108 kDa



      The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.

    References

    ab64838 has not yet been referenced specifically in any publications.

    Publishing research using ab64838? Please let us know so that we can cite the reference in this datasheet.

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