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    rin3-antibody-ab64838.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Regulators
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Anti-RIN3 antibody (ab64838)

  • Datasheet
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Abpromise

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Find out more.

Western blot - Anti-RIN3 antibody (ab64838)

    Key features and details

    • Rabbit polyclonal to RIN3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-RIN3 antibody
      See all RIN3 primary antibodies
    • Description

      Rabbit polyclonal to RIN3
    • Host species

      Rabbit
    • Specificity

      ab64838 detects endogenous levels of total RIN3 protein.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide derived from an internal sequence of human RIN3.

    • Positive control

      • HUVEC and K562 cell extracts.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

      Without Mg2+ and Ca2+
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • Small G Proteins
      • Regulators
      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • Regulation

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    Our Abpromise guarantee covers the use of ab64838 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).

    Target

    • Relevance

      RIN3 belongs to the RIN family of Ras interaction-interference proteins, which are binding partners to the RAB5 small GTPases.
    • Cellular localization

      Cytoplasm. Cytoplasmic vesicle. Early endosome. Note: Activation of tyrosine phosphorylation signaling induces translocation to cytoplasmic vesicles.
    • Database links

      • Entrez Gene: 79890 Human
      • Omim: 610223 Human
      • SwissProt: Q8TB24 Human
      • Unigene: 326822 Human
      • Alternative names

        • DKFZp762H1613 antibody
        • FLJ11700 antibody
        • FLJ22439 antibody
        • Ras and Rab interactor 3 antibody
        • Ras interaction/interference protein 3 antibody
        see all

      Images

      • Western blot - Anti-RIN3 antibody (ab64838)
        Western blot - Anti-RIN3 antibody (ab64838)
        All lanes : Anti-RIN3 antibody (ab64838) at 1/500 dilution

        Lane 1 : HUVEC cell extract
        Lane 2 : K562 cell extract
        Lane 3 : HUVEC cell extract with immunising peptide

        Predicted band size: 108 kDa
        Observed band size: 108 kDa



        The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab64838? Please let us know so that we can cite the reference in this datasheet.

      ab64838 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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