Anti-RING finger protein unkempt like antibody - N-terminal (ab155197)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-RING finger protein unkempt like antibody - N-terminal -
Description
Rabbit polyclonal to RING finger protein unkempt like - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Xenopus tropicalis
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Immunogen
Recombinant fragment, corresponding to a region within N terminal amino acids 1-222 of Human RING finger protein unkempt like (Uniprot ID: Q9H9P5).
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Positive control
- HeLa whole cell lysate; HeLa cells
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General notes
Keep as a concentrated solution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab155197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 81,75, 32, 25 kDa. |
Target
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Function
May participate in a protein complex showing an E3 ligase activity regulated by RAC1. Ubiquitination is directed towards itself and possibly other substrates, such as SMARCD2/BAF60b. Intrinsic E3 ligase activity has not been proven. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the unkempt family.
Contains 4 C3H1-type zinc fingers.
Contains 1 RING-type zinc finger. -
Domain
Although this protein contains a RING domain, intrinsic E3 ligase activity has not been proven (PubMed:20148946). -
Post-translational
modificationsIsoform 4 is ubiquitinated in the C-terminal. Ubiquitination is enhanced by activated RAC1. The presence of the RING finger domain is not essential for ubiquitination to occur. -
Cellular localization
Cytoplasm. Nucleus. Isoform 4 is primarily localized in the cytoplasm but has the ability to shuttle between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 64718 Human
- Entrez Gene: 74154 Mouse
- Entrez Gene: 100144682 Xenopus tropicalis
- SwissProt: Q96EV1 Human
- SwissProt: Q96RZ1 Human
- SwissProt: Q9H9P5 Human
- SwissProt: Q5FWH2 Mouse
- Unigene: 643536 Human
see all -
Alternative names
- C16orf28 antibody
- FLJ12623 antibody
- FLJ23360 antibody
see all
Images
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Western blot - Anti-RING finger protein unkempt like antibody - N-terminal (ab155197)Anti-RING finger protein unkempt like antibody - N-terminal (ab155197) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 81,75, 32, 25 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-RING finger protein unkempt like antibody - N-terminal (ab155197)Immunofluorescent analysis of methanol-fixed HeLa cells labeling RING finger protein unkempt like with ab155197 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
References
This product has been referenced in:
- Avet-Rochex A et al. Unkempt is negatively regulated by mTOR and uncouples neuronal differentiation from growth control. PLoS Genet 10:e1004624 (2014). Read more (PubMed: 25210733) »
