Key features and details
- Rabbit polyclonal to RING finger protein unkempt like - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RING finger protein unkempt like antibody - N-terminal
DescriptionRabbit polyclonal to RING finger protein unkempt like - N-terminal
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Xenopus tropicalis
Recombinant fragment, corresponding to a region within N terminal amino acids 1-222 of Human RING finger protein unkempt like (Uniprot ID: Q9H9P5).
- HeLa whole cell lysate; HeLa cells
General notesKeep as a concentrated solution.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 81,75, 32, 25 kDa.|
FunctionMay participate in a protein complex showing an E3 ligase activity regulated by RAC1. Ubiquitination is directed towards itself and possibly other substrates, such as SMARCD2/BAF60b. Intrinsic E3 ligase activity has not been proven.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the unkempt family.
Contains 4 C3H1-type zinc fingers.
Contains 1 RING-type zinc finger.
DomainAlthough this protein contains a RING domain, intrinsic E3 ligase activity has not been proven (PubMed:20148946).
modificationsIsoform 4 is ubiquitinated in the C-terminal. Ubiquitination is enhanced by activated RAC1. The presence of the RING finger domain is not essential for ubiquitination to occur.
Cellular localizationCytoplasm. Nucleus. Isoform 4 is primarily localized in the cytoplasm but has the ability to shuttle between the nucleus and the cytoplasm.
- Information by UniProt
- C16orf28 antibody
- FLJ12623 antibody
- FLJ23360 antibody
Anti-RING finger protein unkempt like antibody - N-terminal (ab155197) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 81,75, 32, 25 kDa
12% SDS PAGE
Immunofluorescent analysis of methanol-fixed HeLa cells labeling RING finger protein unkempt like with ab155197 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
ab155197 has been referenced in 1 publication.
- Avet-Rochex A et al. Unkempt is negatively regulated by mTOR and uncouples neuronal differentiation from growth control. PLoS Genet 10:e1004624 (2014). PubMed: 25210733