Anti-RING finger protein unkempt like antibody - N-terminal (ab155197)
Key features and details
- Rabbit polyclonal to RING finger protein unkempt like - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RING finger protein unkempt like antibody - N-terminal -
Description
Rabbit polyclonal to RING finger protein unkempt like - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Xenopus tropicalis
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Immunogen
Recombinant fragment, corresponding to a region within N terminal amino acids 1-222 of Human RING finger protein unkempt like (Uniprot ID: Q9H9P5).
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Positive control
- HeLa whole cell lysate; HeLa cells
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General notes
Keep as a concentrated solution.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 81,75, 32, 25 kDa. |
Target
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Function
May participate in a protein complex showing an E3 ligase activity regulated by RAC1. Ubiquitination is directed towards itself and possibly other substrates, such as SMARCD2/BAF60b. Intrinsic E3 ligase activity has not been proven. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the unkempt family.
Contains 4 C3H1-type zinc fingers.
Contains 1 RING-type zinc finger. -
Domain
Although this protein contains a RING domain, intrinsic E3 ligase activity has not been proven (PubMed:20148946). -
Post-translational
modificationsIsoform 4 is ubiquitinated in the C-terminal. Ubiquitination is enhanced by activated RAC1. The presence of the RING finger domain is not essential for ubiquitination to occur. -
Cellular localization
Cytoplasm. Nucleus. Isoform 4 is primarily localized in the cytoplasm but has the ability to shuttle between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 64718 Human
- Entrez Gene: 74154 Mouse
- Entrez Gene: 100144682 Xenopus tropicalis
- SwissProt: Q96EV1 Human
- SwissProt: Q96RZ1 Human
- SwissProt: Q9H9P5 Human
- SwissProt: Q5FWH2 Mouse
- Unigene: 643536 Human
see all -
Alternative names
- C16orf28 antibody
- FLJ12623 antibody
- FLJ23360 antibody
see all
Images
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Western blot - Anti-RING finger protein unkempt like antibody - N-terminal (ab155197)Anti-RING finger protein unkempt like antibody - N-terminal (ab155197) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 81,75, 32, 25 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-RING finger protein unkempt like antibody - N-terminal (ab155197)Immunofluorescent analysis of methanol-fixed HeLa cells labeling RING finger protein unkempt like with ab155197 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Protocols
References (1)
ab155197 has been referenced in 1 publication.
- Avet-Rochex A et al. Unkempt is negatively regulated by mTOR and uncouples neuronal differentiation from growth control. PLoS Genet 10:e1004624 (2014). PubMed: 25210733
