Name: Anti-RING1 antibody [8C12F4]
SKU: ab175149

Product name

Anti-RING1 antibody [8C12F4]
See all RING1 primary antibodies
Description

Mouse monoclonal [8C12F4] to RING1
Host species

Mouse
Tested applications

Suitable for: WB, ELISA, Flow Cyt, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment corresponding to Human RING1 aa 79-263.
Database link: Q06587
Positive control
- RING1 recombinant protein; RING1 (aa: 79-263)-hIgGFc transfected HEK293 cell lysate; MOLT4, LNCaP, Hela, HEK-293 and Jurkat cell lysates; Human cervical cancer and Human rectum cancer tissues; HeLa cells.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS

0.5% protein stabilizer
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

8C12F4
Isotype

IgG1
Research areas

Compatible Secondaries
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
Positive Controls
Related Products
- Recombinant Human RING1 protein (ab112348)

Our Abpromise guarantee covers the use of ab175149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/500 - 1/2000. Predicted molecular weight: 42 kDa.
ELISA		1/10000.
Flow Cyt		1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P		1/200 - 1/1000.

Function

Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
Pathway

Protein modification; protein ubiquitination.
Sequence similarities

Contains 1 RING-type zinc finger.
Cellular localization

Nucleus speckle.
Target information above from: UniProt accession Q06587 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6015 Human
- Omim: 602045 Human
- SwissProt: Q06587 Human
- Unigene: 727565 Human
Alternative names
- Ring1A antibody
- E3 ubiquitin-protein ligase RING1 antibody
- Polycomb complex protein RING1 antibody
- Really interesting new gene 1 protein antibody
- RING finger protein 1 antibody
- Ring1 antibody
- RING1_HUMAN antibody
- RING1A antibody
- Rnf1 antibody
- Transcription repressor Ring1A antibody
see all

Western blot - Anti-RING1 antibody [8C12F4] (ab175149)

Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: RING1 knockout HAP1 cell lysate (40 µg)
Lane 3: MOLT4 cell lysate (20 µg)
Lane 4: LnCaP cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab175149 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab175149 was shown to specifically react with RING1 when RING1 knockout samples were used. Wild-type and RING1 knockout samples were subjected to SDS-PAGE. Ab175149 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-RING1 antibody [8C12F4] (ab175149)

Anti-RING1 antibody [8C12F4] (ab175149) at 1/500 dilution + RING1 recombinant protein

Predicted band size: 42 kDa
Western blot - Anti-RING1 antibody [8C12F4] (ab175149)

All lanes : Anti-RING1 antibody [8C12F4] (ab175149) at 1/500 dilution

Lane 1 : HEK293 cell lysate
Lane 2 : RING1 (aa: 79-263)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 42 kDa
Western blot - Anti-RING1 antibody [8C12F4] (ab175149)

All lanes : Anti-RING1 antibody [8C12F4] (ab175149) at 1/500 dilution

Lane 1 : MOLT4 cell lysate
Lane 2 : LNCaP cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HEK293 cell lysate
Lane 5 : Jurkat cell lysate

Predicted band size: 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [8C12F4] (ab175149)

Immunohistochemical analysis of paraffin embedded Human rectum carcinoma tissue labeling RING1 with ab175149 at 1/200 followed by DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [8C12F4] (ab175149)

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling RING1 with ab175149 at 1/200 followed by DAB staining.
Flow Cytometry - Anti-RING1 antibody [8C12F4] (ab175149)

Flow Cytometrical analysis of HeLa cells labeling RING1 with ab175149 at 1/200 (green) compared to a negative control (red).
ELISA - Anti-RING1 antibody [8C12F4] (ab175149)

ELISA using ab175149 at 1/10000.

Flow cytometry protocols
Immunohistochemistry protocols
Western blot protocols

Datasheet

This product has been referenced in:

Meng S et al. Transdifferentiation Requires iNOS Activation: Role of RING1A S-Nitrosylation. Circ Res 119:e129-e138 (2016). Read more (PubMed: 27623813) »

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Anti-RING1 antibody [8C12F4] (ab175149)

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