Validated using a knockout cell line

Anti-RING1 antibody [8C12F4] (ab175149)

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Overview

  • Product name

    Anti-RING1 antibody [8C12F4]
    See all RING1 primary antibodies

  • Description

    Mouse monoclonal [8C12F4] to RING1

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ELISA, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human RING1 aa 79-263.
    Database link: Q06587

  • Positive control

    • RING1 recombinant protein; RING1 (aa: 79-263)-hIgGFc transfected HEK293 cell lysate; MOLT4, LNCaP, Hela, HEK-293 and Jurkat cell lysates; Human cervical cancer and Human rectum cancer tissues; HeLa cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    0.5% protein stabilizer
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from tissue culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    8C12F4

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 42 kDa.
ELISA 1/10000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/200 - 1/1000.

Target

  • Function

    Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Contains 1 RING-type zinc finger.

  • Cellular localization

    Nucleus speckle.
  • Information by UniProt

  • Database links

    • Alternative names

      • Ring1A antibody
      • E3 ubiquitin-protein ligase RING1 antibody
      • Polycomb complex protein RING1 antibody
      • Really interesting new gene 1 protein antibody
      • RING finger protein 1 antibody
      • Ring1 antibody
      • RING1_HUMAN antibody
      • RING1A antibody
      • Rnf1 antibody
      • Transcription repressor Ring1A antibody
      see all

    Images

    • Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
      Western blot - Anti-RING1 antibody [8C12F4] (ab175149)

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: RING1 knockout HAP1 cell lysate (40 µg)
      Lane 3: MOLT4 cell lysate (20 µg)
      Lane 4: LnCaP cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab175149 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.

      ab175149 was shown to specifically react with RING1 when RING1 knockout samples were used. Wild-type and RING1 knockout samples were subjected to SDS-PAGE. Ab175149 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
      Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
      Anti-RING1 antibody [8C12F4] (ab175149) at 1/500 dilution + RING1 recombinant protein

      Predicted band size: 42 kDa

    • Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
      Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
      All lanes : Anti-RING1 antibody [8C12F4] (ab175149) at 1/500 dilution

      Lane 1 : HEK293 cell lysate
      Lane 2 : RING1 (aa: 79-263)-hIgGFc transfected HEK293 cell lysate

      Predicted band size: 42 kDa

    • Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
      Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
      All lanes : Anti-RING1 antibody [8C12F4] (ab175149) at 1/500 dilution

      Lane 1 : MOLT4 cell lysate
      Lane 2 : LNCaP cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HEK293 cell lysate
      Lane 5 : Jurkat cell lysate

      Predicted band size: 42 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [8C12F4] (ab175149)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [8C12F4] (ab175149)

      Immunohistochemical analysis of paraffin embedded Human rectum carcinoma tissue labeling RING1 with ab175149 at 1/200 followed by DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [8C12F4] (ab175149)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [8C12F4] (ab175149)

      Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling RING1 with ab175149 at 1/200 followed by DAB staining.

    • Flow Cytometry - Anti-RING1 antibody [8C12F4] (ab175149)
      Flow Cytometry - Anti-RING1 antibody [8C12F4] (ab175149)

      Flow Cytometrical analysis of HeLa cells labeling RING1 with ab175149 at 1/200 (green) compared to a negative control (red).

    • ELISA - Anti-RING1 antibody [8C12F4] (ab175149)
      ELISA - Anti-RING1 antibody [8C12F4] (ab175149)

      ELISA using ab175149 at 1/10000.

    References

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    Publishing research using ab175149? Please let us know so that we can cite the reference in this datasheet.

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