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  1. Link

    ring1-antibody-8c12f4-ab175149.pdf

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Epigenetics and Nuclear Signaling Chromatin Remodeling Polycomb Silencing PRC1
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Validated using a knockout cell line

Anti-RING1 antibody [8C12F4] (ab175149)

  • Datasheet
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Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
  • Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
  • Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
  • Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [8C12F4] (ab175149)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [8C12F4] (ab175149)
  • Flow Cytometry - Anti-RING1 antibody [8C12F4] (ab175149)
  • ELISA - Anti-RING1 antibody [8C12F4] (ab175149)

Key features and details

  • Mouse monoclonal [8C12F4] to RING1
  • Suitable for: WB, ELISA, Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

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Overview

  • Product name

    Anti-RING1 antibody [8C12F4]
    See all RING1 primary antibodies
  • Description

    Mouse monoclonal [8C12F4] to RING1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant fragment corresponding to Human RING1 aa 79-263.
    Database link: Q06587

  • Positive control

    • RING1 recombinant protein; RING1 (aa: 79-263)-hIgGFc transfected HEK293 cell lysate; MOLT4, LNCaP, Hela, HEK-293 and Jurkat cell lysates; Human cervical cancer and Human rectum cancer tissues; HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    0.5% protein stabilizer
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    8C12F4
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Polycomb Silencing
    • PRC1

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human RING1 protein (ab112348)
  • Related Products

    • Recombinant Human RING1 protein (ab112348)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/2000. Predicted molecular weight: 42 kDa.
ELISA
1/10000.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P
1/200 - 1/1000.
Notes
WB
1/500 - 1/2000. Predicted molecular weight: 42 kDa.
ELISA
1/10000.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P
1/200 - 1/1000.

Target

  • Function

    Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
  • Cellular localization

    Nucleus speckle.
  • Target information above from: UniProt accession Q06587 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6015 Human
    • Omim: 602045 Human
    • SwissProt: Q06587 Human
    • Unigene: 727565 Human
    • Alternative names

      • Ring1A antibody
      • E3 ubiquitin-protein ligase RING1 antibody
      • Polycomb complex protein RING1 antibody
      • Really interesting new gene 1 protein antibody
      • RING finger protein 1 antibody
      • Ring1 antibody
      • RING1_HUMAN antibody
      • RING1A antibody
      • Rnf1 antibody
      • Transcription repressor Ring1A antibody
      see all

    Images

    • Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
      Western blot - Anti-RING1 antibody [8C12F4] (ab175149)

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: RING1 knockout HAP1 cell lysate (40 µg)
      Lane 3: MOLT4 cell lysate (20 µg)
      Lane 4: LnCaP cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab175149 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.

      ab175149 was shown to specifically react with RING1 when RING1 knockout samples were used. Wild-type and RING1 knockout samples were subjected to SDS-PAGE. Ab175149 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
      Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
      Anti-RING1 antibody [8C12F4] (ab175149) at 1/500 dilution + RING1 recombinant protein

      Predicted band size: 42 kDa

    • Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
      Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
      All lanes : Anti-RING1 antibody [8C12F4] (ab175149) at 1/500 dilution

      Lane 1 : HEK293 cell lysate
      Lane 2 : RING1 (aa: 79-263)-hIgGFc transfected HEK293 cell lysate

      Predicted band size: 42 kDa

    • Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
      Western blot - Anti-RING1 antibody [8C12F4] (ab175149)
      All lanes : Anti-RING1 antibody [8C12F4] (ab175149) at 1/500 dilution

      Lane 1 : MOLT4 cell lysate
      Lane 2 : LNCaP cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HEK293 cell lysate
      Lane 5 : Jurkat cell lysate

      Predicted band size: 42 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [8C12F4] (ab175149)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [8C12F4] (ab175149)

      Immunohistochemical analysis of paraffin embedded Human rectum carcinoma tissue labeling RING1 with ab175149 at 1/200 followed by DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [8C12F4] (ab175149)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [8C12F4] (ab175149)

      Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling RING1 with ab175149 at 1/200 followed by DAB staining.

    • Flow Cytometry - Anti-RING1 antibody [8C12F4] (ab175149)
      Flow Cytometry - Anti-RING1 antibody [8C12F4] (ab175149)

      Flow Cytometrical analysis of HeLa cells labeling RING1 with ab175149 at 1/200 (green) compared to a negative control (red).

    • ELISA - Anti-RING1 antibody [8C12F4] (ab175149)
      ELISA - Anti-RING1 antibody [8C12F4] (ab175149)

      ELISA using ab175149 at 1/10000.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (2)

    Publishing research using ab175149? Please let us know so that we can cite the reference in this datasheet.

    ab175149 has been referenced in 2 publications.

    • Sriramkumar S  et al. Platinum-Induced Ubiquitination of Phosphorylated H2AX by RING1A Is Mediated by Replication Protein A in Ovarian Cancer. Mol Cancer Res 18:1699-1710 (2020). PubMed: 32801161
    • Meng S  et al. Transdifferentiation Requires iNOS Activation: Role of RING1A S-Nitrosylation. Circ Res 119:e129-e138 (2016). PubMed: 27623813

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