Recombinant Anti-RING1 antibody [EPR13047] (ab180170)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13047] to RING1
- Suitable for: IHC-P, WB, ICC/IF, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-RING1 antibody [EPR13047]
See all RING1 primary antibodies -
Description
Rabbit monoclonal [EPR13047] to RING1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Gorilla -
Immunogen
Synthetic peptide within Human RING1 aa 150-250 (Cysteine residue). The exact sequence is proprietary.
Database link: Q06587 -
Positive control
- Human endometrial carcinoma and pancreas tissues. Ramos, MOLT4, LNCaP and HeLa whole cell lysate (ab150035). HeLa cells.
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General notes
Mouse and Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13047 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
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ICC/IF |
1/50 - 1/100.
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IP |
1/30.
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Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
ICC/IF
1/50 - 1/100. |
IP
1/30. |
Target
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Function
Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-type zinc finger. -
Cellular localization
Nucleus speckle. - Information by UniProt
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Database links
- Entrez Gene: 462604 Chimpanzee
- Entrez Gene: 101143477 Gorilla
- Entrez Gene: 6015 Human
- Omim: 602045 Human
- SwissProt: A2T6X5 Chimpanzee
- SwissProt: Q5TJF3 Dog
- SwissProt: A1YER5 Gorilla
- SwissProt: Q06587 Human
see all -
Alternative names
- Ring1A antibody
- E3 ubiquitin-protein ligase RING1 antibody
- Polycomb complex protein RING1 antibody
see all
Images
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RING1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug with ab180170 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab180170 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/10000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug
Lane 2: ab180170 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab180170 in mouse cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [EPR13047] (ab180170)
Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling RING1 using ab180170 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: RING1 knockout HAP1 cell lysate (20 µg)
Lane 3: MOLT4 cell lysate (20 µg)
Lane 4: LnCaP cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab180170 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.ab180170 was shown to specifically react with RING1 when RING1 knockout samples were used. Wild-type and RING1 knockout samples were subjected to SDS-PAGE. ab180170 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-RING1 antibody [EPR13047] (ab180170) at 1/5000 dilution
Lane 1 : Ramos (human Burkitt's lymphoma) whole cell lysate
Lane 2 : Molt-4 (human acute lymphoblastic leukemia) whole cell lysate
Lane 3 : LnCaP (human prostat carcinoma) whole cell lysate
Lane 4 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration 5% NFDM/TBST.
Exposure Time:
Lane 1-3: 3 minutes;
Lane 4: 1 minute -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [EPR13047] (ab180170)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling RING1 using ab180170 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescence analysis of HeLa cells labeling RING1 using ab180170 at 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab180170 has not yet been referenced specifically in any publications.